Synthesis of Silver Nanoparticles Using Turmeric Infused and Antifungal Activity Against Malassezia furfur ATCC 14521
Abstract
Malassezia furfur is a lipophilic fungus and part of the normal flora of the skin. Under certain conditions, Malassezia furfur can cause Pityriasis versicolor (Tinea versicolor). Pityriasis versicolor can generally be treated with azole antifungals. However, recently it has been widely known that Malassezia furfur has experienced resistance. Therefore, a nanoparticle approach was sought. This research aims to synthesize and characterize turmeric infused silver nanoparticles, as well as test the antifungal activity of silver nanoparticles against Malassezia furfur. This research method begins with preparation of turmeric infusion, synthesis of silver nanoparticles using turmeric infusion and characterization using a UV-Vis spectrophotometer, and testing the antifungal activity of silver nanoparticles with ratios of 1:2, 1:4, and 1:6 at a stirring time of 3 hours, 6 hours, and 24 hours using the well method. The results showed that the color of the solution changed from yellow to yellowish brown. Characterization of silver nanoparticles using a UV-Vis spectrophotometer obtained the highest absorbance value at a ratio of 1:6 with a stirring time of 24 hours with a wavelength of 393nm. The antifungal activity test showed that the highest inhibition zone was at a ratio of 1:6 with a stirring time of 6 hours with an average diameter of the inhibition zone of 8.17mm and the lowest was at a ratio of 1:6 with a stirring time of 24 hours with an average diameter of the inhibition zone of 2.78mm. Turmeric infusion can be used as a bioreduction in the synthesis of silver nanoparticles.
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