Klinikal Sains : Jurnal Analis Kesehatan

KEJADIAN INFEKSI NEMATODA USUS DAN HUBUNGANNYA DENGAN PHBS DAN STATUS GIZI PADA PENDUDUK YANG TINGGAL DI DAERAH TEMPAT PEMBUANGAN AKHIR SAMPAH

Sun, 11 Jun 2023 11:50:04 +0700

Landfill (TPA) is a place to dispose of waste and waste to minimize the impact of waste on human health and the environment. People who live around the landfill are at risk of experiencing health problems. The main factor in transmitting STH worm eggs to the human body is poor hygiene. Cleanliness in question is personal hygiene, cleanliness of the food consumed and also the cleanliness of the environment where you live and work. The most common cause of this class of worms is Soil Transmitted Helminths (STH), which are intestinal worms that are transmitted through the soil. This study aims to see incident of nematode infectioan and the relationship between helminthiasis infection with nutritional status and personal hygiene behaviours. It was an observational study with cross-sectional design. This type of research is an observational study with a cross-sectional design. The sample are 93 responden at Sumompo Landfill were taking by purposive sampling. The results found that 24,8% respondent had worm infections with Ascaris lumbricoide,Strongyloises stercoralys and Anchylostoma duodenale. The results of statistical tests on the relationship between worm infection with healthy living behavior and nutritional status showed p>0.05. The conclusion are 25.8% respondents had worm infection; and there is no significant relationship between worm infection with clean and healthy living behavior, and nutritional status.

DETEKSI EKTOPARASIT DAN ENDOPARASIT PADA KUCING PELIHARAAN (FELIS DOMESTICUS) DI WILAYAH TAMBAKSARI, SURABAYA

Anindita Riesti Retno Arimurti, Vella Rohmayani, Dita Artanti, Ruspeni Daesusi — Sun, 11 Jun 2023 11:55:39 +0700

Domestic Cats (Felis domesticus) are favorite mammals that are mostly kept by humans. One of the obstacles in keeping cats is that they can be infected with ectoparasites and endoparasites. Many ectoparasite and endoparasite infections are zoonotic, so the cat's hygiene and health must be maintained by the owner. This study aims to determine the presence of ectoparasite and endoparasite infections in domestic cats in the Tambaksari area of Surabaya.The sample in this study were 32 cats that were kept in the Tambaksari area, Surabaya. This research was conducted on 32 domestic cats in the Tambaksari area of Surabaya. The examination was divided into 2, namely the examination of ectoparsites by means of which were carried out on the body surface, starting from the head, body, legs, and tail. While the examination of endoparasites by means of examination of feces by flotation method using saturated NaCl. The results of the examination were 21.87% positive for infection by ectoparasites, 78.12% were not infected with ectoparasites. The types of ectoparasites that infect are the Felicola subrostarus and the Ctenocephalides felis. While the results of endoparasite examination, as many as 9.37% were positively infected by endoparasites, 90.63% were not infected with endoparasites. Types of endoparasites found eggs of Toxocara spp. and Ancylostoma spp. So ectoparasites and endoparasites infections in domestic cats in the Tambaksari area of Surabaya are quite low. Cat owners should pay more attention to the cleanliness and health of their domestic cats

Keyword: Ectoparasite, Endoparasite, Cat, Felicola subrostarus, Ctenocephalides felis, Toxocara spp., Ancylostoma spp.

PERBEDAAN KABAR HEMOGLOBIN PADA PASIEN ANEMIA SEBELUM DAN SESUDAH VAKSIN COVID 19

Nugroho Tristyanto, Edi Suriaman — Sun, 11 Jun 2023 11:58:50 +0700

Anemia is a condition in which there is a decrease in hemoglobin, erythrocyte and hematocrit levels so that the number of circulating erythrocytes and hemoglobin levels cannot fulfill their main function. Anemia that does not get treatment will result in hypoxia and lead to organ dysfunction, especially breathing. It is this organ dysfunction that makes COVID-19 disease worse. Governments must be prepared to ensure access to and distribution of COVID 19 vaccines on a large and equitable scale if and when a safe and effective vaccine becomes available. Until this article was written in 2022, the total number of confirmed cases from around the world was 476 million cases with 6.1 million confirmed deaths due to COVID-19 infection to the latest variant. The research design is an analytic research with a cross-sectional data collection approach. The data sources used in this study were primary data obtained from before and after hemodialysis to determine the patient's hemoglobin level and secondary data obtained from medical records to determine the sample according to the inclusion and exclusion criteria. In this study, the samples to be taken were anemic patients who received the Covid-19 vaccine, a total of 30 patients. it can be seen that the respondents aged 18-65 years or a number of 24 respondents (80%). It is known that the results of the study found differences in hemoglobin levels before and after the covid - 19 vaccine from 30 patients on examination of the hemoglobin level values before the covid - 19 vaccine obtained the highest hemoglobin level value of 13.3 g/dL and the lowest hemoglobin level value of 7.0 gd /L with an average obtained of 8.66 gd/L while in the description of the value of hemoglobin levels after the covid-19 vaccine the highest hemoglobin level value was 13.5 g/L and the lowest hemoglobin level value was 7.0 gd/L with the average obtained was 9.10 g/dL

UJI IN VIVO TIKUS WISTAR DENGAN BIT MERAH (Beta vulgaris L ) UNTUK PERBAIKAN KELAINAN PROFIL DARAH AKIBAT PAPARAN PESTISIDA

Kartika - Ikawati — Sun, 11 Jun 2023 12:01:45 +0700

Pesticides increase the number of free radicals, triggering oxidative stress causing inflammation and blood profile abnormalities such as anemia, agranulocytosis, neutropenia and thrombopenia. One of the plants that is rich in antioxidants and nutrients to form blood cells is red beet (Beta vulgaris L). The purpose of this study was to determine the ability of red beet (Beta vulgaris L) to improve blood profile abnormalities due to pesticide exposure through in vivo test of Wistar male rats. This research is a laboratory experimental type with a Post Test Only Randomized Control Group Design. The sample of the study was 25 male Wistar rats which were randomly divided into 5 different treatment groups. Mice were exposed to 140 ml/day of pesticides and various doses of red beet ethanol extract for 14 days. The blood profile parameters examined were hemoglobin level, hematocrit, erythrocyte count and leukocyte count. Examination of blood profile parameters manually and automatically. Data analysis with ANOVA and Post Hoc tests. The results of the study proved that the ethanol extract of red beetroot was able to improve blood profile damage due to pesticide exposure in the form of a significant increase in hemoglobin levels, hematocrit values, erythrocyte counts and reduced leukocyte counts from leukocytosis (P<0.05). The optimal dose of red beet ethanol extract is 400 mg/kgBB. Red beets are able to improve blood profile abnormalities due to exposure to pesticides. Red beets are recommended as a non-pharmacological alternative to improve blood profile abnormalities due to exposure to pesticides.

KELARUTAN KALSIUM BATU GINJAL DALAM FRAKSI ETIL ASETAT, N-HEKSANA DAN AIR DARI EKSTRAK ETANOL BUAH TAKOKAK (Solanum torvum Swartz)

Armydha Dwi Susanti, Alip Sofiyatun Nisa — Sun, 11 Jun 2023 12:03:10 +0700

Kidney stones are caused by calcium salts in the urine. Takokak fruit (Solanum torvum Swartz) contains chemical compounds of flavonoids, alkaloids, tannins, saponins, quinones and steroids. The content of flavonoids in the takokak plant is thought to be used as a decay of kidney stones. This study aims to determine the levels of dissolved calcium in ethanol extract and several fractions of takokak fruit in vitro. The process of soaking kidney stones was incubated at 37⁰C for 5 hours. Dissolved calcium was measured using a Visible spectrophotometer at a λmax of 506.5 nm.

Based on the Ca standard calibration curve, a linear regression equation is obtained y = 0.0162 x + 0.1862 with r2 = 0.9939. In order to obtain the percentage of calcium kidney stones dissolved in the ethanol extract, n-hexane fraction, ethyl acetate fraction and the water fraction of takokak fruit respectively 80.49%; 16.3%; 71.85%, and 33.58%.

From these results it can be concluded that the ethanol extract of takokak fruit dissolves calcium kidney stones the most because it is possible that the flavonoid compounds are more interested in the ethanol extract of takokak fruit compared to the ethyl acetate fraction and the water fraction.

EFEK PENYEMPROTAN PESTISIDA TERHADAP KADAR SGOT DAN SGPT PADA PETANI DI DESA SIMPANG ASAM

iis afriayani, Nindya Nigrum Suwondo, Meri Suzana, herry Hermansyah — Sun, 11 Jun 2023 11:53:39 +0700

Pesticide is materian which used dalam increasing agricultural production. In addition includes toxic materials and dangerous. Pesticide enter into body through some way ie the digestive tract, respiratory tract and skin. Effect that caused if accumulated in long term can cause liver cirrhosis effect an increase in SGOT and SGPT in blood. Several risk factor for impaired liver function experienced by farmer use pesticide i.e age, working period, lenght of work, personal protective equipment, and position farmer against wind direction. Research purposes this is determine the analysis effect used pesticide for content SGOT and SGPT on farmers. Type of research use is associative using primary data. Sampling technique used perposive sampling and number samples use 37 samples. Correlation test in research using Chi-square. Analysis result indicate p value >0,005 there is no relationship between characteristics farmer use pesticide such as age, working periode, use of personal protective equipment, and position farmer on wind direction with content SGOT dan SGPT.

Keywords : Pesticide, Liver, SGOT and SGPT

ANTIOXIDANT TESTS OF ROBUSTA COFFEE (COFFEA CANEPHORA), STRAWBERRY FRUIT (FRAGARIA X ANNANASA), AND THE COMBINATION OF THE BOTH WITH VARIOUS SOLVENTS

Fahreza Maulana, Himmi Marsiati, Muhammad Arsyad — Sun, 11 Jun 2023 11:52:34 +0700

Robusta coffee (Coffea canephora) and strawberries (Fragaria x annanassa) are plants containing of antioxidant. This research is aimed to determine the antioxidant activity of each plant and the combination of both plants. The activities were measured by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method towards the extracts of ethanol, ethyl acetate, and water. The result showed that Robusta coffee beans had a value of IC₅₀ 18,96 ppm on the ethanol extract, IC₅₀27,03 ppm on the ethyl acetate extract, and IC₅₀ 20,13 ppm on the water extract. Strawberries had a value of IC50 173,13 ppm on the ethanol extract, IC50 211,03 ppm on the ethyl acetate extract, and IC50 205,15 ppm on the water extract. The combination of beans and strawberries had a value of IC₅₀ 42,82 ppm on the ethanol extract, IC₅₀55,78 ppm on the ethyl acetate extract, and IC₅₀ 41,51 ppm on the water extract. Besides, Vitamin C as the comparison standard had a value of IC₅₀3,68 ppm. The ANOVA test result showed a value of (p<0.05) which indicated that there were significant differences between samples and particular solvents. On the other hand, phytochemical test indicates strawberries were containing of flavonoids and phenolics.

KADAR BILIRUBIN PASCA FOTOTERAPI PADA BAYI DI RSIA FATMA BOJONEGORO.

Budi - Santosa, Siti Istiqomah — Sun, 11 Jun 2023 11:56:50 +0700

One phenomenon that is still often found in newborns is the occurrence of hyperbilirubinemia. Phototherapy is one treatment to avoid a more fatal condition. The aim of this study was to see the effect of phototherapy on total bilirubin levels in infants with hyperbilirubinemia. The Study used Observational analytic research methods were carried out in a population of infants who experienced jaundice at RSIA Fatma Bojonegoro. The sampling technique is a total population of 110 respondents through medical record data. data in the form of respondent characteristics, phototherapy, and bilirubin levels were presented through univariate and bivariate analysis. The results showed that the bilirubin level in infants with hyperbilirubinemia after phototherapy decreased by an average of 3.086 mg/dL. Nonparametric statistical test results with 2 related samples obtained a significance degree value of ρ (0.000) < α (0.05) Ha was accepted, meaning that there was an effect of phototherapy on reducing total bilirubin. levels in babies. Based on the result stydy, that phototherapy has a significant effect on reducing total bilirubin levels in hyperbilirubinemia infants.

UJI EFEKTIVITAS EKSTRAK DAUN TREMBESI (SAMANEA SAMAN) SEBAGAI LARVASIDA PADA LARVA AEDES AEGYPTI

Risnawati Risnawati, Rizki Perdani, Hajrah Hidriya — Sun, 11 Jun 2023 11:54:41 +0700

Eradicating the larvae is one of the vector controls for Aedes aegypti. One alternative way to eradicate the larvae is by using vegetable insecticides (WHO, 1997 in Basri and Farasda, 2019). This study aims to determine the effectiveness of trembesi leaf extract (Samanea saman) as a larvicide on Aedes aegypti larvae. This study used an experimental design in the laboratory with trembesi leaves which were extracted using the maceration method for 4 days (96 hours) using a 96% ethanol solvent. The extract concentrations used were 0.1%, 0.5%, 1%, 5% and 10%. Each treatment contained 20 Aedes aegypti mosquito larvae and was observed every 24 hours and 48 hours. Based on the results of the exploratory test on the effectiveness of trembesi leaves (Samanea saman) against Aedes aegypti larvae with concentrations of 0.1%, 0.5%, 1%, 5%, and 10% which were observed for 24 hours and 48 hours, the results showed that the extract trembesi leaves (Samanea saman) with concentrations of 5% and 10% could kill all larvae populations within 24 hours, while concentrations of 0.5% and 0.1% could kill all larvae within 48 hours. Effectiveness of Trembesi Leaf Extract (Samanea saman) which affects the concentration factor with the most effective concentration at a concentration of 5% which can kill 100% of Aedes aegypti larvae within 24 hours.

PERBANDINGAN TOKSISITAS ANTARA EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura) DAN DAUN KAPAS (Gossypium hirsutum) TERHADAP LARVA UDANG (Artemia Salina Leach) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

Mega Pratiwi Irawan, Alfin Surya — Sun, 11 Jun 2023 11:59:58 +0700

Gossypium hirsutum cotton is a member of the Malvaceae family, and its seeds and leaves have been widely used for health care. G. hirsutum cotton has secondary metabolites in the form of flavonoids, tannins, saponins and steroids that can provide pharmacological effects. This study aims to determine the comparison of the toxicity between the ethanol extract of cherry leaves (Muntingia calabura) and cotton leaves (Gossypium hirsutum) using the BSLT (Brine Shrimp Lethality Test) method. The extract was made by maceration method using 96% ethanol as solvent. This toxicity test was carried out using 48 hours old Artemia salina L. shrimp larvae. The results of this study were analyzed using a probit table with the LC50 value of the ethanolic extract of cherry leaves, which was 100 ppm, while the LC50 value of the ethanolic extract of cotton leaves was 158 ppm. This indicates that the extract is highly toxic to the mortality test of Artemia salina L. larvae

PENGARUH WAKTU PENUNDAAN PADA PEMERIKSAAN KIMIA URIN METODE CARIK CELUP DENGAN SUHU PENYIMPANAN 2-8oC

Wed, 14 Jun 2023 09:01:57 +0700

Changes in urine composition may occur in a specimen that allowed to remain unpreserved at room temperature for longer than 2 hours, without preservatives. Urine received in the laboratory often cannot be directly examined because the number of samples is not comparable to laboratory facilities or because urine collection is carried out at the patient's home or doctor's practice. So that urine is often delayed examination time. This study aims to determine the effect of the delay in urine samples stored at a temperature of 2-8oC on the results of urine chemistry examination using the strip-dip method. This type of research is a quasi-experimental research type. The samples used were 50 respondents who were determined by Quota sampling which were then examined for urine chemistry immediately, a delay of 1 hour, 2 hours, and 3 hours at a storage temperature of 2-8^oC. Data analysis using Kruskal Wallis test.The results found there was an effect of delay time on urine chemistry examination using the stripping method on the results of leukocyte examination with a significant level value of 0.001 which means (p <0.05) and no effect on the results of the examination of nitrite, urobilinogen, protein, pH, blood, specific gravity, ketones, bilirubin and glucose.