UJI DAYA HAMBAT EKSTRAK ETANOL PROPOLIS LEBAH TRIGONA ITAMA TERHADAP SALMONELLA TYPHI
Kata Kunci:
Propolis, Trigona itama, Antibakteri, Salmonella typhi, kloramfenikol.Abstrak
Lebah Trigona merupakan lebah asli Asia yang tidak memiliki sangat dan menghasilkan propolis. Propolis digunakan untuk menutup sel-sel ruang heksagonal pada sarang lebah, komponen utama dari propolis adalah resin (flavonoid, asam fenolat dan ester) atau getah tanaman yang dikumpulkan oleh lebah. Propolis bermanfaat sebagai antioksidan, antimikrba, anti-inflamasi, anti-pendarahan, anti-tumor, antihistamin, antijamur, menguatkan pembuluh darah, dan antivirus. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol propolis lebah Trigona itama terhadap Salmonella typhi, dengan metode difusi cakram pada media MHA dengan kloramfenikol sebagai kontrol positif dan DMSO sebagai kontrol negatif. Ekstraksi dilakukan dengan metode maserasi mengunakan etanol 96%. Ekstrak yang didapat di encerkan menjadi 80%, 60%, dan 40% menggunakan DMSO dengan pengenceran bertingkat. Hasil penelitian menunjukkan terhadap zona hambat ekstrak etanol propolis lebah Trigona itama terhadap Salmonella typhi dengan konsentrasi 80%, 60%, dan 40% dengan rata-rata 14,44 mm, 12,28 mm, dan 10,83 mm, dan pada pembanding sebesar 24,31 mm.
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