JOPS (Journal Of Pharmacy and Science)

MSC Secretome's Antibacterial Activity on P. aeruginosa Isolated from Diabetic Ulcer Patient

2024-06-30T23:08:10+07:00

The study aims to determine the antibacterial activity of adipose tissue-derived Mesenchymal Stem Cell (MSC) secretome against P. aeruginosa bacteria isolated from diabetic ulcer patients. Bacteria were isolated from specimens obtained from diabetic ulcer patient and identified using MacConkey and Blood Agar media followed by Polymerase Chain Reaction (PCR) molecular identification. Confirmed P. aeruginosa bacteria were used to evaluate the MSC secretome's antibacterial activity via the Kirby-Bauer method. Isolated bacteria grew on MacConkey media as gram-negative, non-lactose-fermenting bacteria, showing hemolytic capabilities on Blood Agar media. PCR identification yielded positive results for P. aeruginosa. In antibacterial activity testing against isolated bacteria, MSC secretome at concentrations of 1.25%, 2.5%, 5%, and 10% exhibited efficacy with average inhibitory zones measuring 8.17 mm, 8.23 mm, 8.52 mm, and 9.30 mm, respectively. The MSC secretome demonstrates antibacterial activity against P. aeruginosa bacteria isolated from diabetic ulcer patients.

Overview of Acute Respiratory Infection (ARI) Medication Pediatric Patients at the Puskesmas Sidomulyo, Pekanbaru City

2024-06-30T23:08:10+07:00

Infeksi Saluran Pernapasan Akut (ISPA) adalah penyakit infeksi yang menyerang salah satu bagian atau lebih dari saluran napas, mulai dari hidung (saluran atas) hingga alveoli (saluran bawah). Infeksi saluran pernapasan akut adalah infeksi yang disebabkan oleh virus dan bakteri. Penelitian ini bertujuan untuk mengetahui gambaran penggunaan obat infeksi saluran pernapasan akut (ISPA) pada pasien pediatri di puskesmas sidomulyo rawat jalan kota pekanbaru. sampel yang digunakan yaitu data pasien pediatri ISPA puskesmas pada tahun 2021 di Puskesmas Sidomulyo Rawat Jalan Kota Pekanbaru. Metode yang digunakan retrospektif dengan teknik purposive sampling. Berdasarkan hasil penelitian yang di lakukan, di dapatkan hasil penggunaan antihistamin 165 obat (31,79%), ekspektoran 156 obat (30,05%), analgetik 103 obat (19,84%), antibiotik 87 obat (16,76%), mukolitik 5 obat (0,96%), bronkodilator 2 obat (0,38%), dan kortikosteroid 1 obat (0,19%). Berdasarkan zat aktif obat didapatkan, CTM 165 obat (31,79%), guafenesin 156 obat (30,05%), paracetamol 103 obat (19,84%), amoxicillin 83 obat (15,99%), ambroxol 5 obat (0,96%), cefadroksil 3 obat (0,57%), salbutamol 2 obat (0,38%) dan eritromisin 1 obat (0,19%).

Correlation of Oral Antidiabetics to Creatinine Clearance Value in Type 2 Diabetes Mellitus Patients at a Public Hospital in Jakarta

2024-06-30T23:08:11+07:00

Diabetes mellitus can cause various complications such as stroke, coronary heart disease, peripheral artery disease, retinopathy, neuropathy and nephropathy. Choosing the right drug in diabetes mellitus patients is very important because most antidiabetic drugs are excreted through the kidneys, so it is necessary to monitor creatinine clearance values as an indicator of impaired kidney function. The aim of this research is to find out the relationship between the use of oral antidiabetics and creatinine clearance values in type 2 diabetes mellitus. This research is an observational analytical study with a cross-sectional data collection method on patients type 2 diabetes mellitusbetween January 2023 - December 2023 at a public hospital in Jakarta. This study obtained 39 patients, including the majority of patients who used oral anti-diabetics were women, 56.4%.The largest age was in the range 46 – 55 years at 38.5%. The most common type of drug is a combination of two drugs glimepirid + metformin as much as 35.9% and the creatinine clearance value in type 2 diabetes mellitus patients is a klirens kreatinin value < 90 mL/min/1.73m2 of 66.7%. The results of the chi-square test obtainedp=0.093. Based on the values obtained, there was no significant relationship between the use of oral antidiabetics and creatinine clearance values.

Factors Related to Blood Pressure Control of Hypertensive Patients Using Captopril and Amlodipine

2024-07-06T01:03:46+07:00

Objective: Captopril and amlodipine are the antihypertensive drugs most widely used in primary health care facilities to control blood pressure in hypertensive patients, however, blood pressure control is influenced by many factors other than drug use. This study aims to analyze the differences in the effectiveness of captopril and amlodipine as well as factors related to controlling blood pressure in hypertensive patients

Methods: The research design used cross-sectional correlational analysis using accidental sampling at two community health centers in the city of Palembang. Data sources are secondary data (medical records) and primary data (MMAS-8 questionnaire). Effectiveness is assessed by reducing systolic and diastolic blood pressure. Data were analyzed using descriptive statistics, Mann-Whitney and chi-square tests.

Results: The research subjects were 94 people. The difference in the effectiveness of captopril and amlodipine using the Mann-Whitney test showed p=0.625 for systolic blood pressure and p=0.916 for diastolic blood pressure. The results of the chi-square test showed that the factors age (p=0.035), presence of comorbidities (p=0.015), accuracy of drug selection (p=0.009), medication compliance (p=0.011) were related to blood pressure control, while the gender factor (p=0.418 ), degree of hypertension (p=0.095), duration of therapy (p=0.074) and type of medication (p=0.216) were not related to blood pressure control.

Conclusion: There was no difference in the effectiveness of captopril and amlodipine in this study. Factors related to blood pressure control are age, presence of comorbidities, choice of medication and adherence to medication

The Effect of Ethanol Extract of Cherry Tomato (Solanum lycopersicum L.) on Mast Cell Degranulation in Male White Mice Invitro

2024-06-30T23:08:11+07:00

Cherry tomatoes (Solanum lycopersicum L.) belong to the Solanaceae family which contains antioxidant compounds such as carotenoid compounds, which come from the terpenoid group, for example lycopene, besides cherry tomatoes also have flavonoid compounds. Both of these compounds have many pharmacological activities, one of which is as an anti-inflammatory. Inflammation starts from the process of mast cell degranulation, which is the process of releasing mast cell granules containing inflammatory mediators such as histamine, leukotriene and prostaglandins that cause type I hypersensitivity (allergies). This study aims to determine the effect of cherry tomato ethanol extract on mast cell degranulation of male white mice in vitro. The experimental animals used were male white mice that were actively sensitized after being induced with 20% ovalbumin antigen 0.2 mL/20 g BW on the first day intraperitoneally and the 7th day subcutaneously. The results of the average percentage of mast cell degranulation by cherry tomato extract at concentrations of 25 μg/ml; 50 μg/ml; 100 μg/ml and 200 μg/ml were 54.70%; 51.71%; 46.73%; 42.65% and 38.92%. This study shows that cherry tomato extract (Solanum lycopersicum L.) can inhibit mast cell degranulation in male white mice

Tyrosinase Inhibition and Antioxidant Activity Testing of Mulberry Roots Extract (Morus alba) with Green Extraction Method as Brightening Skin Agent

2024-07-06T01:09:41+07:00

Morus alba (mulberry) root contains a secondary metabolite of oxyresveratrol with its potential skin-brightening activity. The application of the green extraction method using Ultrasonic-Assisted Extraction with Natural Deep Eutectic Solution (NADES-UAE) was developed because of its advantages, namely it can increase extract yields, low toxicity profile, reduce extraction time, shorter extraction stages without evaporation, and can be applied directly in topical preparations for cosmetic products. To ensure the potential for skin-brightening activity, some activity tests on mulberry root extract are needed. The purpose of this study was to determine the in vitro skin-brightening activity of mulberry root extract using NADES-UAE method. The method used was an in vitro tyrosinase enzyme inhibition test using a microplate reader and an antioxidant activity test using the DPPH method. The IC50 value of the best mulberry root extract (glycerin: urea 1:3 for 15 minutes) showed tyrosinase inhibition activity was 178.43 μg/mL. While the IC50 value in the best antioxidant activity test was 1392.14 μg/mL. Tyrosinase inhibitory and antioxidant activities in three samples showed the best results having the highest oxyresveratrol content. NADES-UAE mulberry root extract has the potential as a cosmetic preparation for skin lightening and has antioxidant effects for skin health.

Evaluation of Antibiotic Use Based on ATC/DDD Criteria In Children with Pneumonia at RSI Siti Rahmah Padang

2024-07-06T01:12:04+07:00

Pneumonia is inflammation or infection of the respiratory tract which causes pain and makes it difficult for sufferers to breathe because there is fluid in the form of pus in the alveoli sacs. Pneumonia is the highest cause of death in the world, especially in children under 5 years of age. Pneumonia caused by bacterial infections is treated using antibiotics. Antibiotics that are used inappropriately can cause bacterial resistance, resulting in worsening infections, an increase in infections, longer hospital stays, and increased health service costs. The aim of this research is to determine the quantitative evaluation of antibiotic use in pediatric patients with pneumonia at RSI Siti Rahmah Padang. This research is observational analytic with a retrospective design. The samples used in this study were pediatric pneumonia patients aged ≤18 years who cmet the inclusion criteria. Quantitative analysis was carried out by calculating the quantity of antibiotics used by pediatric pneumonia patients based on ATC/DDD criteria. The research results showed that the total DDD was 51.69 DDD/100 patient-days with the highest DDD value for the antibiotic ceftriaxone with a value of 19.44 DDD/100 patient-days.

The Impact of Educational Videos on the Use of Health Supplements During the Covid-19 Pandemic on the Level of Community Knowledge in Binawidya Sub-District of Pekanbaru

2024-07-06T01:33:11+07:00

The significant increase in confirmed cases of COVID-19 necessitates the implementation of preventive measures by the general public to mitigate the risk of contracting the disease. One strategy that can be employed involves the consumption of health supplements. Among the most prevalent health supplements consumed by the public during the COVID-19 pandemic is vitamin C, which is taken to enhance immunity as part of the preventive measures against COVID-19. This research aims to see the effect of providing educational videos on the use of health supplements during the COVID-19 pandemic on the level of community knowledge in the Binawidya sub-district of Pekanbaru. This research is a quasi-experimental research with a non-randomized control group pretest-posttest design with data collection techniques, namely purposive sampling using a Google form questionnaire. In this research, the sample consisted of individuals from the Binawidya sub-district who met the inclusion criteria, totaling 100 respondents. The analysis of the knowledge data revealed a significant impact of educational videos on the utilization of health supplements amidst the COVID-19 pandemic, as evidenced by an obtained P value of 0.000, which is less than the predetermined significance level of 0.05.

The Demineralization Optimization for Chitosan Synthesis from Crab Shell Waste (Portunus pelagicus)

2024-06-30T23:08:11+07:00

Chitosan, gelatin, albumin, and sodium alginate are examples of natural polymers that are often utilized as a basis material for polymeric nanoparticles. The deacetylation of chitin molecules produces the formation of chitosan. Chitin, protein, CaCO3, MgCO3, and astaxanthin pigment are all found in crab shells. Crab shell is an undervalued potential waste. Despite the fact that copious crab shell waste can be used to produce raw materials and industrial products. According to the findings of Mohadi et al (2014), chitosan was extracted with a yield of 70.71% and a deacetylation degree of 76.6%. Previous research on optimizing chitosan synthesis has involved changing the base reagent at the deacetylation stage. The results showed that a 50% concentration of KOH reagent produced the best chitosan properties. Another study on the synthesis of chitosan from crab shells got a yield of 70.4%, and the following analysis showed that this high yield is due to the amount of calcium. Therefore, demineralization in the synthesis of chitosan from crab shells must be optimized. The calcium content was measured after optimization with various solvent concentration variations. The best demineralization optimization results use 3 M hydrochloric acid with a decrease in calcium content of 97.75%.

Assessment of the Appropriateness of Antituberculosis Drug Utilization in Pulmonary Tuberculosis Patients

2024-06-30T23:08:11+07:00

Tuberculosis, caused by the bacterium Mycobacterium tuberculosis, primarily affects the lungs. This study aims to evaluate the precise use of antituberculosis drugs, focusing on factors such as patient suitability, correct indication, drug selection, dosage, administration method, and treatment duration. Sampling involved Purposive Sampling of pulmonary tuberculosis outpatients Dr. RSUD. H. Abdul Moeloek Bandar Lampung from January to December 2021, resulting in 89 patients. The data showed a majority of male patients, with 52 (58.4%) aged between 36 and 45 years, and 24 (26.9%) weighing between 46 kg and 55 kg. Additionally, 50 patients (56%) tested positive for sputum BTA and had undergone category I treatment previously. Evaluation of antituberculosis drug usage in these outpatients revealed 100% accuracy in patient suitability, indication, drug selection, dosage, and administration method, with a treatment duration of 15 days. Hence, it can be inferred that the utilization of antituberculosis drugs is appropriate.

Effectiveness Test of Ethyl Acetate Fraction Eleutherine Bulbosa for Total Cholesterol, Diastolic-Systolic Blood Pressure Decrease in Male White Rats

2024-07-06T06:34:40+07:00

Eleutherine Bulbosa is one of the plants cultivated by the community for traditional medicine. It's like lowering hypercholesterolemia. Hypercholesterolemia is a condition characterized by high levels of cholesterol in the blood. Besides, it can also lower hypertension. Hypertension is a condition in which blood pressure exceeds the normal limit, with systolic pressure above 140 mmHg and diastolic blood pressure above 90 mmHg. The study aims to look at the effectiveness of the Eleutherine Bulbosa ethyl acetate fraction against the reduction in total cholesterol levels, systolic blood pressure, and diastolic blood pressure in male white rats. Animal cholesterol levels were raised using MLT for 22 days. Cholesterol parameters were tested using a Dumolab DC20 photometer. The inducer used to raise blood pressure is 8% NaCl for 14 days. Blood pressure is measured using non-invasive blood pressure. (NIBP). The results of the research showed that the flavonoid groups that play a role in lowering high cholesterol levels are the anthocyanins and quercetin, while the alkaloid compounds play a part in lowering high blood pressure. Data analysis showed significant results (p<0.05). It can be concluded that the administration of the ethyl acetate fraction Eleutherine Bulbosa is capable of lowering high cholesterol levels and high systolic-diastolic blood pressure.

Evaluation of Antibiotic Use in patients at Pekan Heran Community Health Center using the Anatomical Theraupetic Chemical/Defined Daily Dose and Drug Utilization Method

2024-07-06T01:46:31+07:00

Antibiotics are one of the highest used drugs in the world. Relatively high use of antibiotics can increase the risk of antibiotic resistance. Based on several studies in Indonesia, the use of antibiotics in several Public health center shows the high use of antibiotics. This study aims to find out how antibiotics are used at the Pekan Heran health center in 2019-2022 using the ATC/DDD method and to see changes in the profile of antibiotic use based on the 90% DU profile which is calculated in units of DDD/1000 KPRJ. In this study, there were 8 drugs used at Pekan Heran Public Health Center, namelyamoxicillin, clindamyycin, erythromycin, cefixime, contrimoxazol, metronidazole, chloramphanicol and cefadroxil. Data collection was obtained from the Pekan Heran Public health center. The data processing uses the ATC / DDD method which is processed using Microsoft Excel. The results show that the quantity of antibiotic use has always fluctuating during the 2019-2022 period. The highest use of antibiotic drugs was in 2019 amounting to 708,233 DDD / 1000 KPRJ, and the smallest use was in 2021 amounting to 1,157 DDD / 1000 KPRJ. The profile of the use of antibiotic drugs at the Pekan Heran Public health centerfor the period 2019-2022 was based on DU90%, namely amoxicillin, erythromycin and metronidazole. Amoxicillin is the highest drug used with a percentage in 2019, namely 48,006%, in 2020 amounting to 58,054%, in 2021 it was 27,072%, in 2022 it was equal to 28,437%

A Impact of Potentially Hepatotoxic Drug Use On Clinical Outcomes In Patients With Liver Cirrhosis

2024-08-27T06:07:36+07:00

Liver cirrhosis is a pathological liver disease characterized by the formation of fibrous tissue and regenerative nodules in liver cells. The potential for hepatotoxicity due to the use of hepatotoxic drugs leads to worsening of the liver disease suffered by these patients. This study aims to assess the impact of using potentially hepatotoxic drugs on patients' clinical outcomes. This study was an observational study with a cross-sectional design. Data were collected retrospectively through the medical records of patients with liver cirrhosis hospitalized in 2021. The clinical outcomes studied were SGOT, SGPT, albumin, total bilirubin and length of hospitalization. The results obtained were tested using the Kruskal-Wallis statistical test. A total of 62 patients with liver cirrhosis met the inclusion criteria as the study sample. The number of potentially hepatotoxic drug prescriptions received by patients based on Likelihood scores with categories A, B, C, D and E was 368 drugs out of 776 total drug prescriptions (47.4%). The most commonly prescribed potentially hepatotoxic drugs were paracetamol, ceftriaxon, and levofloxacin. It can be concluded that potentially hepatotoxic drugs are still prescribed to patients with liver cirrhosis. Age, gender, child pugh-score, number of drugs, and number of potentially hepatotoxic drugs had no significant effect (p>0.1) on SGOT, SGPT, albumin and total bilirubin. However, patients who received more drugs would have a higher.

Effect of Variations in Cetyl Alcohol and Cera Alba on the Physical Properties of Virgin Coconut Oil Lotion

2024-07-06T07:33:53+07:00

One of the cosmetics that is widely used topically is lotion, which consists of two phases that do not mix, and can be stabilized using an emulsifying agent. Cetyl alcohol and cera alba have properties as softeners, emulsifiers and absorb water which were chosen as emulsifying agents because they can improve the texture of the preparation. The aim of this research was to see the effect of varying concentrations of cetyl alcohol and cera alba as emulsifying agents in VCO lotion preparations. The research design in this study was pre-experimental with the lotion formula divided into 4 sample groups with different concentrations of cetyl alcohol and cera alba. Physical property tests carried out include organoleptic tests, pH tests, homogeneity tests, spreadability tests and lotion type. The results showed that variations in the concentration of cetyl alcohol and cera alba did not show differences in organoleptic, homogeneity, pH, spreadability and type of lotion for all formulas. It can be concluded that in making VCO lotion with varying concentrations of cetyl alcohol and cera alba it does not affect the physical properties of the lotion

Analysis of Calcium Levels in Maman and Fermented Maman (Cleome gynandra L)

2024-07-06T08:05:17+07:00

Calcium is an important micronutrient that helps prevent stunting. It plays a crucial role in bone growth. Maman (Cleome gynandra L.) is a local plant that grows in the Riau province and is used by local people as a fermented food. To utilize this local plant, it is necessary to analyze its nutrients, including calcium levels. Calcium analysis was carried out using a quantitative descriptive method with an atomic absorption spectrophotometer. Validation of the atomic absorption spectrophotometry method yielded a correlation coefficient (r) of 0.9997, with calcium levels in maman leaves and fermented maman being 3132.33 mg/g and 1976.31 mg/g, respectively.

Novel Finding of Pineapple Peel Extract as Antidiabetic Potential on Rats Induced With Streptozotocin

2024-07-06T16:55:25+07:00

Diabetes mellitus is chronic and metabolic disease which occurs because of the increase of blood glucose level or hyperglycemia caused the body which cannot produce or use insulin effectively. Hyperglycemia has a glucotoxic effect resulting in an increase in ROS (reactive oxygen species) thus inducing liver pathogenesis which promotes severe inflammatory and apoptotic responses, as indicated by an increase in the SGPT enzyme. The pineapple peel contains flavonoid. It is known of having a strong antioxidant activity. With the giving of pineapple peel extract, it is expected to decrease the blood glucose level. This research to find out the the effect of pineapple peel extract (Ananas comusus L. Merr) on decreasing blood glucose levels and SGPT levels in male white rats (Rattus norvegicus) induced by streptozotocin. This research is an experimental study with a research design of pretest and posttest control group design. The samples consisted of 18 rats divided into 6 groups consisted of: normal control, negative control, and positive control group given metformin, and pineapple peel extract with dose 200mg/KgBW, 300mg/KgBW and 500mg/KgBW for 28 days and the measurement of the rats blood glucose was conducted by blood glucose test meter at day 1, day 4 (after post inductionof STZ 45mg/KgBW given by intraperitoneal), and day 29. The analysis result of One Way ANOVA showed that pineapple peel extract with dose 200mg/KgBW, 300mg/KgBW, and 500mg/KgBW decrease the blood glucose level significant (p-value<0,05) with negative control group. There was no significant difference between 200mg/KgBW, 300mg/KgBW and 500mg/KgBW compared with positive control (p>0.05). There was an effect of pineapple peel extract administration on the SGPT levels of STZ-induced rats with a dose of 200mg/kg BW, 300mg/kg BW, 500mg/kg BW (p-value <0.05). The administration of 300mg/kg BW pineapple peel extract had the highest decrease in SGPT levels compared to other groups. Pineapple peel extract (Ananas comosus L. Merr) can reduce blood glucose leveland SGPT levels in rats induced by streptozotocin with the greatest reduction at dose 300mg/KgBW.

The Effect of Giving Melinjo Leaves (Gnetum gnemon L.) Methanol Extract on the Pain Response in Male White Mice Using the Chemical Stimulation Method

2024-07-06T17:17:21+07:00

Nyeri merupakan perasaan sensoris serta emosional tidak menyenangkan, berhubungan dengan kerusakan jaringan. Daun melinjo salah satu tanaman yang bermanfaat sebagai obat tradisional untuk mengobati nyeri. Penggunaan obat tradisional efek sampingnya lebih rendah dibandingkan obat kimia. Penelitian ini bertujuan untuk mengetahui aktivitas analgetik dan dosis terbaik ekstrak metanol daun melinjo pada mencit yang diinduksi asam asetat 1%. Metode penelitian ini merupakan penelitian eksperimental dengan subjek penelitiannya mencit sebanyak 25 ekor yang dibagi 5 kelompok perlakuan dengan masing-masing kelompok terdiri 5 ekor mencit. Kelompok perlakuan terdiri kontrol negatif CMC-Na 1%, kontrol positif ponstan, kelompok ekstrak metanol daun melinjo dosis 50, 100, 150 mg/kgBB, penginduksi nyeri diberikan asam asetat 1%. Pengamatan dilakukan dengan mengamati geliat mencit untuk dihitung % proteksinya. Data kemudian diuji statistik One Way Anova dan Post-Hoc LSD atau Games-Howell. Hasil ekstrak metanol daun melinjo dengan dosis 150 mg/kgBB memiliki efek analgetik paling baik karena memiliki % proteksi 78,04%, serta memberikan efek yang signifikan pada mencit yang diiinduksi asam asetat 1%. Ekstrak metanol daun melinjo memiliki efek analgetik terbaik pada mencit secara signifikan (p<0,05) dan nilai mean differencenya lebih besar.

Analysis of Oral Albumin Use and Factors Affecting the Success of its Therapy

2024-07-06T17:19:08+07:00

Hypoalbuminemia is a risk factor for medical treatment. Albumin has been shown to be a predictor in a number of studies in various settings. Several studies examined albumin as a marker of nutritional status and its association with outcomes (eg, mortality and length of hospital stay). In critical illness, the Acute Physiology and Chronic Health Evaluation (APACHE III) risk score includes serum albumin levels and shows a strong inverse correlation between serum albumin and mortality in critically ill patients.

This research was conducted observationally to analyze the use of oral albumin and the factors that influence the success of therapy in the Lung Inpatient Room at Dr Soetomo Regional Hospital for the period 1 January – 31 October 2022. Patients diagnosed with Community Pneumonia, Lung Cancer and Lung Tuberculosis. This research was carried out by collecting secondary data via e-medical records. Data taken were patient demographic data (name, gender, weight, age, length of stay) and albumin data before and after receiving oral albumin.

From the research results, the number of samples was 87. The number of samples was 87 patients. Of the 87 patients, 66 male patients (75.86%) were 21 female patients (24.14%), the largest age range was 51 - 60 years, 21 patients (24.14%). The highest body weight ranged from 61-70 kg for 50 patients (57.47%), the longest length of stay ( Length of Stay ) was 10-20 days for 45 patients (51.72%). The most common primary diagnosis was new tuberculosis in 42 patients (48.28%). The highest use of oral albumin was more than 100 capsules, as many as 20 patients (22.99%) with the highest duration of use being 10-19 days, namely 38 patients (43.68%). The statistical results show that there is no significant difference between before receiving oral albumin and after receiving oral albumin with a value of P = 0.541. Factors that influence the success of oral albumin therapy are length of stay with a value of P = 0.001 *

Keywords: Oral albumin, pulmonary tuberculosis, lung cancer, pneumonia, length of stay

Skrining Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Kunyit Hitam (curcuma caesia Roxb) Dengan Metode DPPH (1,1 diphenyl-2-picrylhydrazyl)

2024-07-06T17:38:47+07:00

Black turmeric (Curcuma caesia Roxb) comes from the Zingberaceae family and is one of the natural antioxidant plants capable of combating free radicals. This study aims to determine the secondary metabolite compounds and antioxidant activity test of black turmeric ethanol extract. Extraction was carried out using 96% ethanol solvent by maceration method, secondary metabolite analysis by phytochemical screening method, and antioxidant activity test using DPPH (1,1 diphenyl-2-picrylhydrazyl) method with ascorbic acid as a comparator. The instrument used was a microplate reader with a maximum measurement wavelength of 520 nm. The parameter used to determine antioxidant activity is IC50 (Inhibition concentration). In this study, the results of phytochemical screening test on black turmeric positively contained alkaloids, flavonoids, tannins, saponins, steroids, and triterpenoids. The antioxidant activity test results of black turmeric ethanol extract (Curcuma caesia Roxb) as a sample obtained an IC50 value of 124.33 ppm, while ascorbic acid as a comparator had an IC50 value of 2.49 ppm. Based on these IC50 values, black turmeric ethanol extract has moderate antioxidant activity and ascorbic acid has very strong activity.

Utilization of Methanol Extract Sea Pandan Fruit (Pandanus tectorius) in Hand and Body Cream as an Antioxidants

2024-07-06T23:15:27+07:00

Sea pandanus is a coastal plant with great potential in the cosmetic industry due to its rich contains phenolic and flavonoid compounds that are abundant in the fruits. The purpose of this research is determine the antioxidant activity of sea pandanus fruit and to evaluate the result of hand and body cream formulation. Pandanus was chosen because it is not widely explored.The result of study showed that the methanol extract of sea pandanus contains phenolic, flavonoid, saponin, and steroid compounds. The antioxidant activity in the hand and body cream formulation was 33,183 µg/mL, indicating strong antioxidant activity below the IC50. The high level antioxidant activity from the methanol extract of sea pandanus can be used in the formulation of hand and body cream. Characteristics in the quality assessment of all the hand and body cream did not meet the requirements.

Inhibitory Activity Test of α-amylase Methanol , n-Hexane Fraction and Ethanol Extract of Moringa Leaves (Moringa oleifera L)

2024-07-20T12:02:06+07:00

Diabetes mellitus is a disease characterized by increased blood sugar levels (hyperglycemia). One of the enzymes that plays an important role in breaking down oligosaccharides and disaccharides into monosaccharides so that they are ready for absorption is the α-amylase enzyme which can delay and lengthen the digestion time of carbohydrates. Moringa (Moringa oleifera L) is a plant that originates from India and has now spread widely to tropical and subtropical areas throughout the world. Moringa leaves are known to contain the flavonoid, β-sitosterol, which can reduce sugar levels. The aim of this research was to examine inhibitory activity of α-amylase on methanol, n-hexane fraction and ethanol extract of Moringa leaves. The method for data analysis was carried out by obtaining antidiabetic test data, namely the % inhibition value of the α-amylase enzyme activity. From the results of the research that has been carried out, it can be concluded that there is α-Amylase enzyme inhibitory activity with moderate strength in samples of methanol partition and n-hexane partition of Moringa leaves carried out in vitro, with IC50 values of 105.302µg/ ml and 158.29µg/ml. Meanwhile, theMoringa leaves ethanol extract sample was carried out in vitro, with an IC50 value of 81,74 µg/ml.