JOPS (Journal Of Pharmacy and Science)

Hair-Growth Promoting Activity of Ethanol Extracs of Green Okra (Abelmoschus esculentus L.) in Male Rabbits

2024-12-31T23:42:36+00:00

Reduced hair density is a hallmark of hair loss, a medical condition. A deficiency in hair nourishment is one of the reasons of hair loss. The compounds in green okra include tannins, alkaloids, and flavonoids, are ingredients that may promote hair development. The purpose of this study was to study the effect of green okra ethanol extract on the hair development of male white rabbits and identify the optimal dose for this purpose is the main objective of this research. This study used a true experimental method. The hair-fertilizing activity test of green okra ethanol extract was carried out on 6 rabbits as positive control, negative control, normal control and samples with extract doses of 5%,10%, 20%. The results of the study showed that the average hair growth of rabbits with concentrations of 5%, 10% and 20% was 12.12 mm, 13.62 mm and 16.06 mm. The results of the anova test were 0.000. The conclusion of this study is that there is an effect of green okra ethanol extract on rabbit hair growth.

Characteristics of 96% Ethanol Extract of Kaffir Lime Peel, GCMS Analysis and SPF Value (Sun Protection Factor)

2024-12-31T23:42:36+00:00

Exposure to high intensity ultraviolet light can cause sunburn, erythema, and tanning. In addition, ultraviolet radiation encourages the formation of ROS (reactive oxygen species) and nitrogen species (RNS), resulting in skin damage. To prevent this, antioxidants are needed which function to protect the skin from the bad effects of ultraviolet rays. Kaffir lime or what is commonly called kaffir lime has the potential to be a rich source of bioactive compounds. Kaffir lime peel contains essential oil components such as linalool, citronellol, geraniol, citronellal, and other components that have antioxidant activity. The aim of this research was to determine the characteristics of 96% ethanol extract of kaffir lime peel, chemical content and determine the SPF value. Kaffir lime peel was extracted using 96% ethanol and tested for extract characteristics, GCMS analysis and SPF value tested using the Mansur method. The results of the extract characteristics test showed a yield of 8.22%, air content of 8.74%, ash content of 2.17% and drying loss of 1.3%. Phytochemical screening showed results containing tannins, alkaloids and flavonoids and secondary metabolite compounds, GC-MS analysis showed the compounds beta-mycrene, gamma terpinene, linalool, terpinen-4-ol, geraniol, caryophyllene, nerolidol, sphatulenol, citronellal and Neral. SPF test results at a wavelength of 290-320nm with a concentration of 100 ppm: 23.096271, 150 ppm: 35.833618, 200 ppm: 45.047732, 250 ppm: 56.55952, 300 ppm: 68.919985

Sociodemographic Factors Associated with Chronic Obstructive Pulmonary Disease Comorbidities: Based on Indonesia Family Life Survey-5

2024-12-31T23:42:36+00:00

COPD is still one of the major health problems in Indonesia. The aim of this study was to look at the relationship between sociodemographics and the incidence of comorbidities in COPD patients. Cross-sectional study design, using IFLS-5 data. Inclusion criteria were patients with a diagnosis of COPD by self-report, and at least 15 years old. Results: Of 34,252 people filling out household health questionnaires in IFLS 5, 618 subjects had COPD. Based on multivariate test, gender (aOR: 1.792 (1.282-2.506); p-value: 0.001), age 36-45 years (aOR: 1.259 (0.764-2.074); p-value: 0.028), age 46-55 years (aOR: 2.418 (1.365-4.282); p-value: 0.002), age 56-65 years (aOR: 2.418 (1.365-4.282); p-value: 0.002), and age >65 years (aOR: 3.502 (1.884-6.510); p-value: 0.000). 002), age 56-65 years (aOR: 3.502 (1.884-6.510); p-value: 0.000), and age >65 years (aOR: 6.508 (3.338-12.690); p-value: 0.000) had a significant association with the incidence of comorbidities in COPD patients in Indonesia.

Isolation and Characterization of Bacteria from Bekasam-Fermented Fish (Rasbora sp) and Their Antibacterial Potential

2024-12-31T23:42:36+00:00

Bekasam is a traditional fermented fish product. This study aimed to isolate and identify the bacteria involved in the fermentation of Bekasam from Riau and evaluate the antibacterial potential of their ethyl acetate extracts. Bacterial isolation was conducted using the pour plate method with selective media (MRS agar-CaCO3 0.5%). Antibacterial activity was tested against Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecalis) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using the agar diffusion method, with chloramphenicol as a positive control. Four bacterial isolates were identified: Lactobacillus sp1 (BAC1), Lactobacillus sp2 (BAC2), Bacillus sp1 (BAC3), and Bacillus sp2 (BAC4). All ethyl acetate extracts from these isolates demonstrated antibacterial activity. The highest activity was observed from the Lactobacillus sp1 extract against E. coli, with an inhibition zone diameter of 16.1 mm.

The Immuno candies; Formulation, Characterization and Antioxidant Activity of Gotu Kola (Centella asiatica (L.) Urb) Herbal Hard Candy

2024-12-31T23:42:36+00:00

Background: Pagagan is a plant that has antioxidant properties. The benefits of antioxidants are essential for everyone because they can prevent cell damage due to the bad influence of free radicals. Formulating gotu kola in the form of candy is one effort so that the antioxidant effects of gotu kola can always be obtained easily because candy is a snack everyone likes and can be obtained easily anywhere. Objective: Formulate, physical characterization and antioxidant activity of hard candy immuno candies. Method: Hard candy formulation with varying concentrations of gotu kola herbal essence (0%, 1%, 5%, 10%, and 15%), physical characterization using organoleptic tests, water content, weight uniformity and stability, and antioxidant activity using the DPPH method. Results: Hard candy made with concentrations of 0%, 1%, 5%, 10%, and 15% has a sweet and slightly bitter taste (typical of gotu kola) at a concentration of 5%-15%, has a typical weak sugar aroma, and highwater content. respectively 0.72%, 0.77%, 0.84%, 0.8%, 0.89%, 0.95%, and is stable at a storage temperature of 8ÂºC-15ÂºC with closed packaging, has good weight uniformity, and IC50 values â€‹â€‹were 32552.69 Âµg/mL, 12992.589 Âµg/mL, 12077.09 Âµg/mL 9669.15 Âµg/mL, 9484.519 Âµg/mL Conclusion: Gotu kola herbal juice can be formulated into hard candy up to a concentration of 15 %, has good physical characteristics as well as inactive antioxidant activity.

Phytochemical Screening of Citronella Extract (Cymbopogon nardus) with Different Solvents and Stability of Mosquito Repellent Lotion

2024-12-31T23:42:36+00:00

Dengue Hemorrhagic Fever (DHF) is a health problem in Indonesia. Until the 22nd week of 2024, there were 119,709 dengue cases with 777 deaths. The use of citronella (Cymbopogon nardus) repellent is done to prevent it. This study aims to determine the content of citronella extract with ethanol (polar), ethyl acetate (semi-polar), and n-hexane (non-polar) solvents as active ingredients of mosquito repellent lotion and determine the stability of the lotion. The type of this research is experimental research with true experimental design:the posttest-only control group design. Phytochemical screening was conducted to determine the saponin, flavonoid, alkaloid, phenolic, triterpenoid, and steroid content of each extract. Lotion stability is tested for 28 days by storing the lotion at room temperature. On days 0, 14, and 28, the organoleptic, homogeneity, pH, adhesion, spreadability, and emulsion type tests were carried out on the lotion. The results of phytochemical screening showed that the ethanol extract contains saponins, flavanoids, and phenolics. The ethyl acetate extract contains steroid. The n-hexane extract contains triterpenoid and steroid. The results of the stability test showed that all preparations met the requirements of the organoleptic test, homogeneity test, pH test, adhesion test, and emulsion type test which are good and stable. But it does not meet the requirements of a good spreadability test.

Effectiveness Test of Ethyl Acetate Fraction Corn Hair (Zea mays L) as an Antihypertensive in Male White Rats

2024-12-31T23:42:36+00:00

Corn hair is a group of stigmas that are smooth, soft, and look like threads or yellowish hair. The use of corn hair, which is a waste from corn cultivation, is still limited to its use as a traditional medicine. Hypertension or high blood pressure is a chronic condition characterized by increased blood pressure on the walls of arteries. This study aims to determine the effect of the effectiveness of the ethyl acetate fraction of corn hair (Zea mays L) on the reduction of blood pressure in male white rats of the Wistar strain. This type of study is an experimental pre-test and post-test control group design, using Wistar male white rat test animals, the inducing used is NaCl 8%. Blood pressure measurements are carried out using the CODAÂ®. Data analysis using the One Way Anova Statistical test followed by the Duncan test obtained the result that the administration of the ethyl acetate fraction of corn hair with doses of 250 mg/kgBB, 500 mg/kgBB, and 1000 mg/kgBB had a significant effect on reducing blood pressure (p<0.05). It can be concluded that the variation in the dose of the ethyl acetate fraction of corn hair (Zea mays L) has a significant difference in the reduction of blood pressure and affects histological improvement. The effective dose of the ethyl acetate fraction of corn hair in lowering blood pressure is a dose of 1000 mg/kgBB.

Formulation and Antibacterial Test of Staphylococcus aureus Against Paper Soap Preparation of Lemongrass Extract (Cymbopogon citratus)

2024-12-31T23:42:36+00:00

Paper soap is a unique soap that has a thin sheet shape similar to paper. Citratus or also known as lemongrass is a well-known herbal medicine with a myriad of benefits. This study aims to evaluate the ethanol extract of lemongrass (Cymbopogon citratus Dc) as an ingredient in making paper soap. Lemongrass is known to contain various compounds such as saponins, flavonoids, and essential oils, which have the potential to inhibit bacterial growth. The experimental method was used to make paper soap from lemongrass extract with formulations I, II, and III with a percentage of 5%, 10% and 15% lemongrass extract. Then the evaluation and test of antibacterial effectiveness against staphylococcus aureus using the paper disc diffusion method. Organoleptic tests showed that the color and aroma of the soap became more intense with increasing extract concentration. The pH test revealed that the soap had a pH range of 6-11, suitable for soap. The foam height test showed all formulas, except the third, met the standard. The antibacterial test showed that the zone of inhibition of Staphylococcus aureus increased as the concentration of lemongrass extract increased. Formulations with 5%, 10%, and 15% percentages formed inhibition of 21.35 mm, 23.52 mm, and 26.6 mm, respectively. These results indicate that formulations containing higher lemongrass extract have relatively better antibacterial ability.

Effect of Ethanol Extract of Japanese Papaya Leaves (Cnidoscolus aconitifolius (Mill.) I.M. Johnst) on Leukocyte Differential of Male White Mice

2024-12-31T23:42:36+00:00

Immunomodulators are medicines that alter or influence the immune system to make it work more effectively. Immunomodulators can increase (immunostimulants) or decrease immune response (immunosuppressants). Secondary metabolites that have immunomodulatory activity are polyphenols, alkaloids, and terpenoids. One of the plants that contain these secondary metabolites is Japanese papaya leaves. Japanese papaya leaves have been widely used as traditional medicine to treat various diseases. This study aims to determine the effect of the administration of Japanese papaya leaf extract on differential leukocytes of male white mice. This study used 24 male white mice with skin allergies after administering 20% ovalbumin 0.2 mL/20g BW intraperitoneally on day 1 and day 7 subcutaneously. Twenty male white mice were divided into 4 groups. Group I (control) mice werenâ€™t given ovalbumin antigen or papaya leaf extract; groups II, III, and IV were given ovalbumin and extract (100; 200; 400 mg/kg BW). After oral administration of the extract for seven days, leukocyte differential count of white mice. Giving ethanol extract of japanese papaya leaves at doses of 100, 200, and 400 mg/kg BW can significantly affect leukocyte differentials, namely lymphocytes and monocytes (p<0.05). While basophils, eosinophils, rod-neutrophils, and segment-neutrophils are not significantly different (p>0.05).

Effect of Vitamin E Administration on Primary Dysmenorrhea in Adolescent Girls in South Sempaja Village

2024-12-31T23:42:37+00:00

Prevalensi dismenorea primer di Indonesia mencapai 64,5%. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian vitamin E terhadap kondisi dismenorea primer pada remaja putri di Kelurahan Sempaja Selatan. Metode penelitian adalah quasi eksperimental dengan time-series design, menggunakan pre-test dan post-test. subjek dalam penelitian ini adalah 50 remaja putri usia 10-19 tahun di Kelurahan Sempaja Selatan. Sampel yang digunakan vitamin E dikonsumsi selama 5 hari yaitu pada 2 hari sebelum menstruasi hingga 3 hari saat perdarahan. Instrumen penelitian berupa kuesioner karakteristik, kuesioner skala nyeri NRS dan WBFPRS dan kuesioner efek selain penurunan nyeri. Hasil data karakteristik remaja putri yang mengalami dismenorea primer di Kelurahan Sempaja Selatan yaitu berusia 19 tahun (38%), usia menarche >12tahun (50%), nyeri karena genetik (33%), dan status BMI underweight (56%). Selanjutnya, didapatkan hasil penurunan intensitas nyeri yang bermakna setelah mengonsumsi vitamin E jika dibandingkan pada saat responden tidak mengonsumsi vitamin E. Intensitas nyeri berdasarkan skala NRS menunjukkan penurunan pada saat responden mengonsumsi vitamin E (p = 0,000) dan berdasarkan skala WBFPRS juga menunjukkan penurunan intensitas nyeri setelah mengonsumsi vitamin E (p = 0,000). Vitamin E juga menimbulkan efek selain penurunan nyeri, yaitu nafsu makan meningkat 19%, kulit menjadi lebih cerah 12%, menjadi lebih bugar 16%, dan pandangan mata kabur 2%. Berdasarkan hasil tersebut, dapat disimpulkan bahwa vitamin E memiliki pengaruh terhadap kondisi dismenorea primer pada remaja putri di Kelurahan Sempaja Selatan
Kata kunci: Dismenorea primer, Efek selain penurunan nyeri, Intensitas nyeri, Vitamin E

Correlation of Drug-Related Problems and Quality of Life in Chronic Kidney Disease (CKD) Patients Undergoing Hemodialysis

2024-12-31T23:42:37+00:00

The complexity of treatment with the disease in patients with chronic kidney disease (CKD) can increase the incidence of Drug Related Problems (DRPs). Analysis of DRPs is very useful to reduce the duration of illness, death, and treatment costs. The condition of patients who require hemodialysis therapy can cause changes such as physical, psychological, lifestyle and social that have an impact on the quality of life. This study aims to determine correlation between DRPs and the quality of life of CKD patients undergoing hemodialysis at Dr. Pirngadi Hospital, Medan, Indonesia.

This study uses an observational method with a retrospective and prospective cross sectional research design on 72 patients who met the inclusion criteria in February-March 2024. DRPs were analyzed applying Cipolle and quality of life was obtained using the EQ-5D-5L questionnaire. The data was analyzed using the Spearman Rho test in the SPSS program.

The results showed that there were DRPs in 64 patients with the categories of needing additional drugs (3.38%), unnecessary therapy (15.54%), drug interactions (35.13%) and ineffective drugs (45.95%). Quality of life with categories of good (36.1%), adequate (41.7%), and poor (22.2%). The patient's average quality of life score was 0.657. There was a correlation between the number of DRPs and the patient's quality of life (r=-0.802) with a significance value of p=0.032 (p<0.05), which shows that the higher the incidence rate of DRPs, the lower the patient's quality of life. Based on the results of the study, it was concluded that there was a significant relationship between the number of DRPs events and the quality-of-life value of CKD patients undergoing hemodialysis.

The Effect of Maceration and Ultrasonic Extraction Methods on the Antioxidant Activity of Beluntas Leaves (Pluchea indica L.)

2024-12-31T23:42:37+00:00

The antioxidant activity of the ethanol extract of beluntas leaves (Pluchea indica L.) was tested using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This method is simple, easy to use and short in time. Several plants have used the ultrasonic extraction method for a short time and produced a higher yield than the maceration method. The aim of this research was to determine the effect of extraction methods on the antioxidant activity of beluntas leaves (Pluchea indica L.). This research method is an experimental method that uses beluntas leaves (Pluchea indica L.), extracted using the maceration method for 24 hours and ultrasonics for 30 minutes, 60 minutes. The extraction results were measured for their antioxidant activity using a UV-Vis Spectrophotometer. The results showed that extraction of beluntas leaves (Pluchea indica L.) using the maceration method produced an IC₅₀ of 79.99 Î¼g/ml and sonication for 60 minutes produced an IC₅₀ of 54.29 ug/ml, including the strong category, while the ultrasonic method for 30 minutes produced an IC₅₀ of 48.81 ug/ml. ml is in the very strong category. The conclusion of this research is that the ultrasonic extraction method produces higher antioxidant activity than the maceration method.

Effectiveness Test of Mahkota Dewa Leaf (Phaleria macrocarpa) Ethanol Extract on Incised Wound in White Rats

2024-12-31T23:42:37+00:00

Mahkota Dewa is a medicinal plant that contains several active substances such as alkaloids, sapoins, tannins which can be used to affect collagen by inhibiting the production of wound tissue. The purpose of this study was to determine the effectiveness of the ethanol extract ointment from the leaves of Mahkota Dewa as a healer for cuts in white rats. This research is an experimental study, designed with a posttest Only Control Group design, where this study used the ethanol extract of Mahkota Dewa leaf ointment in three different concentrations: 5%, 10%, 15%, Povidone Iodine Ointment and Ointment Base. A number of 15 white rats were used as experimental animals. Mahkota Dewa Leaf Ethanol Extract Ointment with a concentration of 5%, 10%, 15% can heal cuts within 8 days, 7 days and 5 days respectively. Ointment with a concentration of 15% had almost the same healing time as the positive control, and the group given the ointment base (negative control) recovered on the 12th day. The conclusion of this study is that the ethanol extract of the leaves of Mahkota Dewa is effective in healing cuts in white rats.

Anticonvulsant Activity of Flower Extract from Telang Plant (Clitoria ternatea L) in Male Albino Mice Induced Strychnine

2024-12-31T23:42:37+00:00

The telang plant (Clitoria ternatea L.) has been reported to have sedative activity and has the potential as an anticonvulsant. The purpose of this study was to test the anticonvulsant activity of the flower extract of the telang plant on male albino mice of the Swiss Webster strain. The extraction process used the maceration method. Testing parameters included seizure onset, time of death and recovery time. The doses used were 100, 200, and 400 mg/kgbw, and diazepam was used as a comparison with a dose of 5 mg/kgbw. The results of this study indicate that the flower extract ot the telang plant at doses of 100, 200, and 400 mg/kgbw has anticonvulsant activity. Increasing the dose of the flower extract of the telang plant correlated very strongly with the prolongation of seizure onset. A dose of 400 mg/kgbw can prevent the death of mice in the convulsion model. The flower extract of the telang plant has the potential to be developed as an anticonvulsant.

Validation Test of Method for Determination of Ibuprofen Level in Tablet Preparations with Trade Name and Generic by UV Spectrophotometry

2025-01-25T16:58:23+00:00

This study aims to determine the levels of ibuprofen in tablet preparations using the UV spectrophotometric method and determine the suitability of ibuprofen tablets with trade and generic names that meet the requirements according to the Indonesian Pharmacopoeia Edition VI. The determination of ibuprofen levels in tablet preparations was tested for validity based on linearity, limit of detection (LOD) and limit of quantitation (LOQ) parameters, accuracy with the spiked-placebo recovery method and precision. Based on the results obtained linearity value of r = 0.9993 with limit of detection (LOD) 0.2825 ppm and limit of quantitation (LOQ) 0.9418 ppm, accuracy obtained at 3 concentrations of 8, 10 and 12 ppm respectively 99.59%; 99.29%; and 99.99% and on precision obtained RSD results of 0.77%; 0.49%; 1.48%. The results of the determination of levels in the four samples of generic tablet preparations and trade names are 100.91%; 104.02%; 100.91%; and 100.99%. From the results of the study, it is concluded that the UV spectrophotometric method used has met the parameters set in the validation test and the ibuprofen levels obtained also meet the requirements set in the Indonesian Pharmacopoeia VI Edition, which is not less than 90% and not more than 110%.

Cost-Effectiveness Analysis of Erythropoietin in Anemia Therapy Strategies for Chronic Kidney Disease Patients Undergoing Hemodialysis: A Systematic Review

2024-12-31T23:42:37+00:00

Chronic kidney disease is a significant health issue, with increasing case numbers leading to higher treatment costs. This condition causes decreased kidney function and can result in anemia due to impaired red blood cell production. This study aims to analyze the cost-effectiveness of anemia treatment using Erythropoietin, either as a single therapy or in combination, for patients with chronic kidney failure undergoing hemodialysis. The methods employed include conducting a systematic review to identify problems, establish inclusion and exclusion criteria, and search various databases for relevant studies. The results show that cost-effectiveness varies depending on the Hemoglobin target and the type of Erythropoietin used. In conclusion, setting an appropriate Hemoglobin target and selecting the correct type of Erythropoietin are crucial for effective anemia treatment. This systematic review recommends a Hemoglobin target of >10â€“11 g/dL and the use of Erythropoietin is adjusted to the patient's condition so that the choice is more cost effective.

Antioxidant Activity of Tetragonula testacetarsis from East Kalimantan

2024-12-31T23:42:37+00:00

Bee pollen is known for its various therapeutic activities, one of which is as an antioxidant. The utilization of Tetragonula testacetarsisbee pollen from East Kalimantan is still limited, and there is little scientific information available about its antioxidant activity. This study aims to determine the compound content in Tetragonula testacetarsis bee pollen and to evaluate its antioxidant activity. The pollen was macerated using 70% ethanol to obtain a thick ethanol extract. The extract was then tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with UV-Vis spectrophotometry, and the antioxidant activity was determined using the % inhibition parameter. The test results showed that the % inhibition of Tetragonula testacetarsis bee pollen was 94.77% at a concentration of 100 ppm, indicating that Tetragonula testacetarsisbee pollen has antioxidant activity.

Iron Content and Hedonic Test of Cookies With Kelakai and Mung Beans Flour

2024-12-31T23:42:37+00:00

Background: To promote local food security, food diversification efforts can be made by utilizing local food sources such as kelakai and mung beans that can be processed into flour as a substitute for wheat flour in making cookies. Objectives: Analyze the iron content and determine the characteristics, organoleptic quality, and acceptability of cookies made from kelakai bean flour and mung bean flour. Methods: Completely Randomized Design with 3 treatments, F1 with a ratio of 40 g mung bean flour and 10 g kelakai flour, F2=40 g : 15 g, and F3=40 g: 20 g. Characteristics and acceptability testing using hedonic test; Fe content using atomic absorption spectophotometry. Results: The cookies produced have the characteristics of brownish green color, unpleasant odor (langu), less sweet taste, and very crunchy texture. The largest Fe content is in formula F3 which is 1.305 mg per piece of cookies (Â± 45 grams). The results of the acceptability test on aroma, taste, color, and texture show that the most preferred cookies are in formula F1. Conclusion: Cookies formula F1 has the potential to be developed into functional food because it has a fairly high iron content and good acceptability.

Evaluation of Antituberculosis Potential of N-Hexane Extract And Ethanol Extract of Soursop Leaves (Annona Muricata L.) In Vitro

2025-01-12T14:40:19+00:00

Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis, primarily affecting the lungs and transmitted through droplets. Indonesia ranks second in the world for pulmonary TB cases. Due to increasing resistance to synthetic TB drugs, alternative treatments are needed, such as soursop leaves, which are known to treat symptoms like coughing and bleeding. This study aims to evaluate the potential of soursop leaf extract against TB. The method used in this study is the Lowenstein-Jensen method with varying concentrations. Sputum samples from TB-positive patients were identified using Zheil-Nelsen staining, while soursop leaf extract was obtained through percolation using n-hexane and ethanol fractions. The results showed that the moisture content of soursop leaf powder was 5%, meeting the simplisia standard. The antituberculosis test revealed resistance to synthetic drugs starting from the second week, while the n-hexane extract showed resistance from the first week. The ethanol fraction inhibited bacterial growth until the third week, but positive growth occurred in the fourth week. Therefore, soursop leaf extract, particularly the ethanol fraction, shows potential as an alternative TB treatment, which warrants further investigation.

Uji Aktivitas Scaffold Kolagen-Kitosan Terhadap Penyembuhan Luka Buatan pada Kulit Tikus (Rattus Norvegicus)

2025-01-18T17:17:44+00:00

Wounds are disorders of the skin's protective function characterized by the loss of epithelial tissue continuity, either with or without damage to other tissues such as muscles, bones, and nerves. Scaffolds with porous structures play an important role in supporting cell function and helping the formation of new tissues or organs. The design of scaffolds for tissue engineering must meet several criteria, such as having a surface that supports cell adhesion, stimulates cell growth, and maintains differentiated cells. In this study, scaffolds were made using chitosan and collagen. Chitosan is known to stimulate cell proliferation, increase collagen formation, and accelerate epithelial cell regeneration in injured skin. Meanwhile, collagen is considered a very promising material in tissue engineering because of its biocompatible, biodegradable, and low antigenicity properties. The results showed that collagen-chitosan scaffolds with added glycerol were able to accelerate wound healing in mouse skin. On the 16th day, the wound healing process was already visible in the scaffold group with a ratio of 80:20:20, while on the 17th day there was no significant difference between the comparison group and the 70:30:20 (F2) and 80:20:20 (F3) scaffold groups. This shows that the better the combination of collagen and chitosan, the faster the wound healing process. Higher collagen concentrations in chitosan scaffolds have been shown to be effective in increasing regeneration and accelerating wound healing.