EFEKTIVITAS PEMBUATAN SEDIAAN TETES TEBAL MALARIA MENGGUNAKAN DARAH K3EDTA DENGAN MODIFIKASI PENAMBAHAN CaCl2

Abstract

Malaria remains a problem in South Kalimantan, with a malaria elimination rate of 53.8%. Accurate diagnosis is crucial for malaria elimination. The malaria diagnostic method utilized is the microscopic examination, which is easy and affordable. This method involves preparing thick and thin blood smears using capillary blood. However, when an anticoagulant is used in the preparation of thick blood smears for malaria, such as blood with anticoagulant, it may cause the blood smear to peel off or not adhere to the glass slide during hemolysis. Calcium chloride (CaCl₂) is one of the reagents used in the hematology laboratory for prothrombin time testing. CaCl₂ can activate platelets in blood with the anticoagulant K₃EDTA to induce clotting. This study aimed to determine the concentration of CaCl₂ that produces a 1.5 cm diameter thick blood smear for malaria. The experimental method was employed for this purpose. CaCl₂ solutions were prepared at concentrations of 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, and 0.1%. Microscopic examination results showed clear lysis of red blood cells, distinct leukocytes, and visible Plasmodium vivax parasites. The ANOVA test yielded a significance value of 0.00, indicating a difference in the diameter of thick blood smears for malaria prepared using blood with anticoagulant reacted with CaCl₂. Based on the Post Hoc test, the concentration of CaCl₂ that yielded favorable results for preparing thick blood smears for malaria was 4%, resulting in a diameter of 1.5 cm