Klinikal Sains : Jurnal Analis Kesehatan

VALIDASI METODE ANALISIS PENETAPAN KADAR SENYAWA ALKALOID PADA EKSTRAK TERIPANG (PARACAUDINA AUSTRALIS) DENGAN METODE SPEKTROFOTOMETRI UV-VIS

Nuril - Jelali — 2024-11-20

Teripang ( Paracaudina australis ) diketahui memiliki aktivitas biologis dan farmakologis karena mengandung banyak senyawa bioaktif, salah satunya adalah alkaloid. Untuk mengetahui kadar alkaloid yang terkandung dalam teripang, maka perlu dilakukan penentuan kadar. Pada penelitian ini, penentuan konsentrasi dilakukan dengan menggunakan analisis instrumental menggunakan metode spektrofotometri UV-Vis. Spektrofotometri UV-Vis merupakan metode analisis yang memanfaatkan panjang gelombang UV dan Visible sebagai daerah serapan untuk mendeteksi senyawa. Tujuan dari penelitian ini adalah untuk melakukan validasi metode analisis penentuan kadar senyawa alkaloid dalam ekstrak teripang menggunakan spektrofotometri UV-Vis. Parameter validasi metode dalam penelitian ini meliputi linearitas, LOD dan LOQ, selektivitas, presisi dan akurasi. Hasil linearitas diperoleh persamaan regresi y = 0,0377x + 0,0635 dengan nilai koefisien korelasi r2 = 0,9992. Nilai LOD yang diperoleh sebesar 0,26 ppm dan nilai LOQ sebesar 0,86 ppm. Selanjutnya terdapat parameter selektivitas yang menunjukkan hasil serapan yang sama antara larutan standar dengan larutan sampel pada panjang gelombang 205 nm dan 273 nm. Hasil pengujian presisi yang dilakukan telah memenuhi persyaratan dengan hasil %RSD yang diperoleh sebesar 2,1%. Parameter akurasi yang diperoleh sebesar 101,68% Â± 0,011, 100,66% Â± 0,008 dan 101,14% Â± 0,003. Hasil identifikasi pada pengujian ini menunjukkan bahwa kandungan alkaloid pada teripang Paracaudina australis sebesar 22,6 ppm Â± 0,16. Kesimpulan dari penelitian ini adalah diperoleh metode pengujian yang valid dengan rata-rata kandungan alkaloid sebesar 22,6 ppm Â± 0,16.

Kata kunci : Alkaloid, Paracaudina australis , spektrofotometri UV-Vis, metode validasi

GAMBARAN NILAI HEMATOKRIT PADA PASIEN TUBERCOLOSIS DENGAN MENGGUNAKAN DARAH VENA DI RUMAH SAKIT UMUM DAERAH LABUANG BAJI MAKASSAR

Rahmat Saleh — 2024-11-20

Tuberculosis is one of the diseases that can be transmitted through the air caused by the bacteria Mycobacterium tubercolosis. The purpose of this study was to determine the hematocrit picture in tuberculosis patients. The method used in this study is descriptive quantitative. The research sample was examined directly using the macrohematocrit method. The procedure in this study includes several stages, namely the pre-analytical, analytical, and post-analytical stages. In the pre-analytical stage, the researcher prepared the tools and materials to be used. Analytical stage, at this stage the researcher took samples and prepared samples, Post-analytical stage, at this stage the researcher calculated the normal hematocrit value. Based on the results of the study, it showed that there were 4 people with low hematocrit values â€‹â€‹and 4 people with high hematocrit values. While the hematocrit value in men had a low hematocrit value of 2 people and a high hematocrit value of 2 people, while in women the hematocrit value was low as many as 2 people and a high hematocrit value of 2 people. In this study, there was no difference in hematocrit values â€‹â€‹between men and women.

UJI AKTIVITAS ANALGESIK EKSTRAK KULIT PISANG KEPOK (Musa paradisiaca L.) PADA MENCIT JANTAN (Mus musculus)

Oktavia Nia Agustin — 2024-11-22

Unpleasant multidimensional sensory experience When tissue is damaged is the meaning of pain. However, the pain can be managed by taking painkillers, but the use of painkillers causes several side effects, one of which is stomach irritation. Banana plants are an alternative that is widely used to produce medicine from natural ingredients. Kepok banana peels are waste that has not been utilized optimally. With 5 groups each consisting of 5 mice, this study used the hot plate method to evaluate the analgesic activity of kepok banana peel extract (Musa paradisiaca L.) in male mice (Mus musculus). These groups included the negative control group (CMC Na 0.5%), positive control group (Diclofenac sodium 0.13 mg/20 gBW), and kepok banana peel extract group (1000 mg/kgBW, 2000 mg/kgBW, and 3000 mg/kgBW). The results of the research showed that kepok banana peel extract (Musa paradisiaca L.) had analgesic activity on male mice (Mus musculus) utilizing the Rapid Heating technique, the % MPE value for Kepok Banana Peel Extract (1000 mg/kg BW) of 19.33%, Banana Peel Extract Kepok (2000 mg/kg BB) of 40.43%, and Kepok Banana Peel Extract (3000 mg/kg BB) of 43.88%. Varying doses of kepok banana peel extract (Musa paradisiaca L.) had an influence on analgesic activity in male mice (Mus musculus) with a p value of 0.001 (<0.05).

Keywords: Analgesic, Hot plate, Mus musculus, Musa paradisiaca L.

ANALISA PEMANTAPAN MUTU INTERNAL TAHAP PRA-ANALITIK PEMERIKSAAN HEMATOLOGI

Bastian Bastian — 2024-12-16

Stages pre-analytic are stages beginning in an inspection laboratory. Errors on stage pre-analytic account for 70% of frequent errors in the laboratory. To anticipate errors, the so-done effort stabilizes quality. To increase quality service and repair quality results. The Objectives: Is to stabilize internal quality during the pre-analytic stage of hematology in the laboratory at House Sick Type C, Palembang. Study this method is descriptive-quantitatvie with a purposive sampling technique in the officer laboratories â€œXâ€ and â€œYâ€, respectively total 15 respondents. Results: In the â€œXâ€ hospital laboratory has an average result of 96% and is said to be good, while in the â€œYâ€ hospital laboratory, it has an average 97% and is said to be good. That there is no significant difference between the second House Sick. Conclusion: Stabilization of internal quality stage pre-analytical inspection hematology on officers in the laboratories of Hospital â€œXâ€ and Hospital â€œYâ€ Palembang can be stated as good.

PERBANDINGAN PERTUMBUHAN Aspergillus niger PADA MEDIA POTATO DEXTROSE AGAR (PDA) DAN MEDIA ALTERNATIF DARI SINGKONG, UBI JALAR, DAN BEKATUL

Indah Lestari — 2024-11-20

Aspergillus niger is one of the fungi that can produce citric acid and is often used in some studies because of its resistance to very low acidic conditions. Potato Dextrose Agar medium is a common medium for culturing fungi in the laboratory. Alternative media are media that use materials from nature whose content is not yet known in detail. Rubber cassava has a carbohydrate content of 98.4674%. The carbohydrate content of white sweet potato is 28.78%. Rice bran has a carbohydrate content of 84.36%. The purpose of this study was to determine the comparison of the growth diameter of Aspergillus niger fungi on Potato Dextrose Agar (PDA) media and alternative media made from cassava, sweet potato, and rice bran. The research used a pure experimental method. Aspergillus niger isolates were observed microscopically, after which they were planted on PDA media and alternative media, incubated at 37Â°C for 72 hours, then the diameter of fungal growth was measured. The results showed that the alternative media that had the largest Aspergillus niger fungal growth diameter compared to PDA media as a control was alternative media from rice bran with the smallest diameter of 40 mm and the largest diameter of 80 mm.

ANALISIS PERBANDINGAN KADAR FLAVONOID TOTAL TEH HIJAU DAN TEH HITAM DENGAN MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS

Antonia Vani Kurniawati — 2024-11-26

Tea leaves (Camellia sinensis) are a functional plant that is useful for preventing cancer, maintaining immunity and preventing premature aging. Flavonoids are one of the largest groups of polyphenols which are active compounds from tea leaves (Camellia sinensis). This study aims to determine the total flavonoid content of green tea leaf extract and black tea leaf extract using 2 extraction methods, namely maceration with 96% ethanol solvent and infusion using water solvent. The method in this research uses qualitative tests (Wilstarter cyanidin and NaOH 10%) and quantitative tests (UV-Vis Spectrophometry) and conclusions will be drawn using the SPSS statistical test T (independent)-test. The results of the qualitative test using the starter cyanidin and 10% NaOH were positive for containing flavonoids due to a reddish color change. In the quantitative test, the assay was carried out using UV-Vis spectrophotometry with a wavelength of 434 nm. In this study, the total flavonoid content obtained in the green tea maceration method was 15.728% w/w Â± 0.209, black tea maceration 13.357% w/w Â± 0.737, green tea infusion 4.153% w/w Â± 0.124 and black tea infusion 3.320% w /b Â± 0.352. There is a significant difference between green tea and black tea using both the infusion and maceration methods as shown by a sig value â‰¤ 0.05.

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK AIR TANAMAN SEMANGGI (Marsilea crenata C.Presl) METODE FRAP (Ferric Reducing Antioxidant Power)

raisya adila adila — 2024-11-20

The clover plant (Marsilea crenata C.Presl) is a plant that is widely used for food and has the potential to be developed as a herbal medicine. Clover plant extract using methanol, chloroform, and ethyl acetate was found to have weak antioxidant activity. The aim of this research was to test the antioxidant activity in air solvent extracts of clover plants using the FRAP (Ferric Reducing Antioxidant Power) method and analyze the effect of variations in extract concentration on the antioxidant activity test. The method in this research is clover plant extraction by maceration using an air solvent. Test antioxidant activity using the FRAP method using a UV-Vis spectrophotometer with a maximum wavelength of 596 nm. The extract yield of 42% showed that it positively contained flavonoids, saponins, polyphenols and a water content of 9.94% Â± 0.1276. The results showed that clover plants had weak antioxidant activity (IC₅₀ 351.04 ppm) compared to vitamin C as a positive control which showed very strong antioxidant activity (IC₅₀ 22.60 ppm). From the results of this study, it can be said that increasing the extract concentration can increase the percentage of inhibition of free radical formation, but the increase is not linear, as indicated by the R² value not approaching 0.99.

MODIFIKASI AMONIUM OKSALAT DENGAN PEWARNA ALAMI BIT MERAH SEBAGAI FORMULA BARU REAGEN HITUNG JUMLAH TROMBOSIT

Kartika - Ikawati — 2024-11-20

Abstract

Platelet count using the Brecker koncrete method with 1% Ammonium oxalate reagent has a weakness that it cannot color platelets so that they are difficult to count. Red beet contains natural dyes that can color platelet organelles. This study aims to prove the ability of Ammonium oxalate modification with natural red beet dye (Beta vulgaris L) as a new formula for platelet count reagents. Method. This type of research is a laboratory experiment with a comparison of platelet counts using 1% Ammonium oxalate reagent and modification of Ammonium oxalate-red beet extract in a ratio of 1: 1, 1: 2, 2: 1 and automatic methods. The object of research is EDTA venous blood from 30 samples of AAK 17 Semarang students taken using the convenience sampling technique. Manual platelet counts using the Brecker concrete method with a counting chamber and an automatic method using a hematology analyzer. The sharpness of platelet staining is assessed under a microscope. The comparison test uses Repeated Anova. The results of the study showed that the color of platelets examined with the Ammonium-bit modification reagent (1: 2) was sharper with an average number approaching the gold standard. The statistical test results obtained sig 0.069 (> 0.05) or no significant difference. Beet extract (Beta vulgaris L) has the potential as an alternative natural dye in manual platelet counts (Brecer koncrite)

keywords : trombosit, red bit, Amonium oksalat

UJI AKTIVITAS ANTIOKSIDAN INFUSA SEMANGGI (Marsilea crenata C. Presl) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-PicrylHydrazyl)

Tiara Juliar Subakti — 2024-11-20

Changes in lifestyle and environment now increase the risk of exposure to free radicals that cause degenerative diseases. Compounds needed to neutralize free radicals are antioxidants. One source of antioxidants is clover (Marsilea crenata C. Presl), which has previously been studied and has shown no antioxidant activity in various extraction solvents, but the clover infusion method with water solvent has not been studied, so the purpose of this study was to determine the antioxidant activity of clover infusion (Marsilea crenata C. Presl). This research method uses the DPPH method, then the antioxidant activity test of clover infusion (10% b/v) with a concentration of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, and vitamin C as a positive control measured by a UV-Vis spectrophotometer. The absorbance value obtained at the maximum DPPH wavelength (516 nm) was used to calculate the linear regression equation of concentration and% inhibition. The results of the study obtained the IC₅₀ value of clover infusion of 804.0 ppm and vitamin C as a positive control of 29.1 ppm. The conclusion of the study is that clover infusion shows no antioxidant activity (IC₅₀ > 500 ppm), while vitamin C shows very strong antioxidant activity (IC₅₀ > 50 ppm). Further research suggestions are related to the development of clover plant extraction methods and solvents (Marsilea crenata C. Presl) and antioxidant activity test methods.

AKTIVITAS ANTIBAKTERI EKSTRAK KULIT MANGGIS (Garcinia mangostana L.) TERHADAP BAKTERI Haemophilus influenzae

Firdha Rachmawati — 2024-11-20

Respiratory tract infections caused by the bacterium Haemophilus influenzae is a significant health concern due to the bacteria's resistance. Mangosteen peel extract (Garcinia mangostana L.) has potential as an antibacterial agent but is rarely applied to H. influenzae. This study aims to evaluate the antibacterial ability of mangosteen peel extract against the growth of H. influenzae. The mangosteen peel was extracted using the maceration method with ethyl acetate, and n-hexane as solvents. Through the diffusion method, ethyl acetate and n-hexane extracts at concentrations of 15; 30; 62,5; and 125 mg/mL were able to inhibit the growth of H. influenzae. The largest inhibition zone was observed at a concentration of 125 mg/mL, with an average diameter of 7,25 Â± 0,07 mm for the n-hexane extract and 8,7 Â± 0,71 mm for the ethyl acetate extract. It can be concluded that n-hexane and ethyl acetate extracts have antibacterial activity against the respiratory infection-causing bacterium H. influenzae, with the highest concentration being 125 mg/mL.

HUBUNGAN PERILAKU HIDUP BERSIH DAN SEHAT (PHBS) DENGAN INFEKSI SOIL TRANSMITTED HELMINTHS (STHs) PADA ANAK SD DI TIWOHO KECAMATAN WORI

Indra Elisabet Lalangpuling — 2024-11-20

One of the factors that causes Soil Transmitted Helminths infection (intestinal nematodes that are transmitted through soil) is Clean and Healthy Living Behavior (PHBS). STHs usually breed in tropical areas with high temperatures and humidity. The aim of this research was to find out whether STHs worm infection which was transmitted through soil in elementary school children in Tiwoho Village, Wori District was related to clean and healthy living behavior (PHBS). This type of research is descriptive with a sampling method, namely purposive sampling of 65 stool samples that meet the inclusion criteria. Stool samples were examined microscopically using the native method, flotation method and Baerman Test method. The Square test was used in data analysis to assess whether there was a relationship between PHBS and STHs worm infection. The results of this study show that there is a relationship between Clean and Healthy Living Behavior (PHBS) and STHs worm infection, especially the habit of washing hands with soap before eating and the habit of biting nails with a p value <0.005.

PERBANDINGAN KADAR HEMOGLOBIN, GLUKOSA DARAH, DAN LEUKOSIT SEBELUM DAN SESUDAH HEMODIALISA PADA PASIEN GAGAL GINJAL KRONIS KOMORBID DAN NON KOMORBID DIABETES MELLITUS DI RSPC

Risma Adzkia Putri — 2024-11-20

Chronic Kidney Disease (CKD) is an impairment of the glomerular organ function of the kidneys. The process of blood purification by collecting waste is called hemodialysis. The prevalence of CKD increased from 1990 to 2017 globally by 21.3% and the mortality rate increased by 41.5%. The purpose of this study was to compare hemoglobin, leukocyte, and blood glucose levels before and after hemodialysis in patients with and without comorbid Diabetes Mellitus (DM) at Pertamina Cilacap Hospital (RSPC). The method used was cross sectional using univariate and bivariate analysis with JASP program. The results showed that in CKD patients without DM, hemoglobin levels were significantly different (p<0.05) after hemodialysis. While leukocyte and glucose levels had no significant difference (p>0.05). In CKD patients with DM, there is a significant difference in hemoglobin levels (p<0.05) after hemodialysis. In addition, there is a significant difference in glucose levels (p<0.05) after hemodialysis. Meanwhile, there was no significant difference in leukocyte levels (p>0.05). Comparison of pre and post hemodialysis between CKD without DM and CKD with DM there is a significant difference (p<0.05) in hemoglobin and blood glucose levels but there is no significant difference (p>0.05) in leukocyte levels.

PERBANDINGAN HASIL PEMERIKSAAN KOLESTEROL TOTAL MENGGUNAKAN SAMPEL SERUM DAN PLASMA EDTA SEGERA DAN DISIMPAN SELAMA 4 JAM

Luvia Isma Hanifah — 2024-11-20

Cholesterol is a lipid substance (fat component) being one among other known components, fat being a nutrient that is fulfilled by the body. The purpose of the study was to determine the comparative results of total cholesterol examination using serum and plasma EDTA immediately and stored for 4 hours. This study is analytic using cross sectional research design. The results showed in statistical tests using the Paired Sample T-Test test on serum samples immediately examined and stored for 4 hours found (p=0.015) so that the results (p>0.05) concluded there was no difference in serum samples. EDTA plasma samples immediately examined and stored for 4 hours found the results (p<0.001), comparison of total cholesterol levels using serum and EDTA plasma samples immediately examined and stored for 4 hours found (p<0.001) so that the results (p<0.05) are concluded there is a difference between total cholesterol levels in EDTA plasma immediately examined and stored for 4 hours and serum and EDTA plasma immediately examined and stored for 4 hours.

Key Words : Cholesterol, Serum, EDTA Plasma, 4 Hour Delay

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT DAUN TIGARON (Crataeva nurvala Buch. Ham) TERHADAP Staphylococcus aureus DENGAN METODE SUMURAN

Putri Kartika Sari — 2024-11-26

Staphylococcus aureus infection is characterized by tissue damage accompanied by purulent abscesses. Antibiotics are a group of drugs using to treat and prevent bacterial infections. However, long-term use causes antibiotic resistance. Efforts made to increase antibiotic resistance include using natural ingredients as alternative treatments. Tigaron is an empirical medicinal plant whose leaves are used to treat abscesses, wounds and skin diseases. This research aims to determine the types of secondary metabolite compounds and antibacterial activity against Staphylococcus aureus from ethyl acetate extract of tigaron leaves (Crataeva nurvala Buch. Ham). This research was experimental with a post test only control group design with 7 concentration variations. Extraction made by maceration using ethyl acetate, identification of secondary metabolite using phytochemical screening, antibacterial activity tested using well diffusion method. The results showed that concentration â€‹â€‹of 0.31 mg/mL, 1.55 mg/mL and 7.75 mg/mL were in medium category. Concentrations of 38.75 mg/mL and 193.75 mg/mL were in strong category. Chloramphenicol as a positive control had strongest inhibition zone diameter. The conclusion are ethyl acetate extract of tigaron leaves contain alkaloid, flavonoid and steroid which inhibiting the growth of Staphylococcus aureus, so it has the potential to be a natural antibiotic using to treat infectious diseases

RESISTENSI ANTIBIOTIK DAN PERTUMBUHAN STAPHYLOCOCCUS AUREUS PADA MEDIA AGAR DARAH

Aldinova Gondo Ruming Asmoro — 2024-11-26

A microorganism growth medium is a material containing nutrients that support the growth and development of microorganisms. This nutrient is called the culture medium, and the growth process is known as culture, which can be enriched by the addition of blood. The aim of the researchers was to examine human blood, sheep's blood and cow's blood as growth media for Staphylococcus aureus bacteria. Is there a difference in the resistance test and growth patterns of Staplococcous aureus bacteria in human blood, sheep's blood and cow's blood media. Growth media in human blood, sheep's blood and cow's blood media. Bacterial growth in human blood media with round-shaped colonies, yellowish white with convex elevation, smooth surface, and medium size on cattle blood with a different type of hemolysis with negative results. smooth, and medium sized on sheep blood media with different types of hemolysis with positive results. Bacterial growth on cow blood media bacterial growth on cow blood media colonies are round, yellowish white with convex elevation, smooth surface, and medium sized on cow blood with hemolysis type which is different from a negative result. conclusion is that the Stapylococcus aureus bacteria has the same inhibitory zone in human blood, sheep blood and cow blood media. There is no difference between human blood, sheep blood and cow blood media. The difference in the type of hemolysis in sheep blood is because it has beta hemolysis type and in human blood, and cow's blood has gamma hemolysis..

Key words: Staphylococcous aureus, growth patterns, blood agar media

PENGARUH JENIS PELARUT EKSTRAKSI TERHADAP KADAR ALKALOID DALAM EKSTRAK TERIPANG (Paracaudina australis) MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS

Aulya Sagita Rahma Sujana — 2024-12-16

Sea cucumbers are a type of marine organism that move slowly and live in shallow waters such as sand, mud, algae, coral reefs where sea cucumbers are a source of animal protein consumed by the public. Research on various types of sea cucumbers has shown that there are several bioactive compounds, one of which is alkaloids. Alkaloids are one of the secondary metabolite compounds that are often found in nature, such as in plant and animal tissues. Previous research results show that there are alkaloids in sea cucumber extract. The aim of this research is to determine how this limited type of solvent impacts the amount of bioactive compounds contained in sea cucumbers (Paracaudina australis). Qualitative and quantitative tests were carried out to test alkaloid compounds. Qualitative tests use color reactions with Dragendroff, Wagner and Mayer reagents. Quantitative tests were carried out using Uv-Vis spectrophotometry with a maximum wavelength of 273 nm. The standard solution used is caffeine. Quantitative analysis showed that all extracts with various solvents were positive for containing alkaloids. The highest alkaloid content is ethyl acetate extract with levels of 71.0116 Â± 0.2872 ppm, then followed by acetone extract which is semipolar with levels of 22.6531 Â± 0.2277 ppm and the lowest is chloroform extract with levels of 13.9622 Â± 0.0283 ppm.

Keywords: Sea cucumber; Alkaloids; Solvent Type; Uv-Vis Spectophotometry

PROFIL LIPID PADA LANJUT USIA SEBAGAI FAKTOR RESIKO PENYAKIT METABOLIK DI KENDARI SULAWESI TENGGARA

Ani Umar — 2024-12-16

Metabolic diseases are a global health problem that is increasing especially in developed countries (Silva et al., 2020). The incidence is higher in the elderly population due to physiological and metabolic changes associated with aging (Silva et al., 2020). Lipid profile is an important indicator in assessing the risk of metabolic diseases. Blood lipids include total cholesterol, LDL (Low Density Lipoprotein), HDL (high Density Lipoprotein), and triglycerides, playing a major role in the development of cardiovascular disease. (Emmanuel et al., 2022) The purpose of this study was to determine the lipid profile in the elderly as one of the metabolic diseases. This study used a cross-sectional design with a target population of 50 elderly individuals aged 60 years and over. data were collected through direct measurement of lipid profiles including total cholesterol, triglycerides, HDL, and LDL using the CHOD-PAP method (Chlorimetric Derivate- Para Amino Phenazone) using a spectrophotometer. The results of the study showed that from 50 elderly patients, the lipid profile levels obtained were total cholesterol levels in the normal category with an average cost of 1.66, where 66% of respondents obtained normal cholesterol levels, while 34% showed high cholesterol levels. in the triglyceride variable, the results obtained were 72% of respondents had normal triglyceride levels, while 28% experienced an increase. HDL levels showed more positive results, with 82% of respondents having good HDL levels, and only 18% having low HDL levels, and, analysis of LDL levels revealed that only 8% of respondents had high LDL levels, while 92% had normal LDL levels in men and women, TG/HDL limits are <150 and> 40 mg/dl, and cholesterol limits <200 and LDL <150.

PENGARUH PENUNDAAN PEMERIKSAAN SPESIMEN URINE DENGAN PENYIMPANAN PADA COOLBOX TERHADAP VIABILITAS BAKTERI

Ni Wayan Desi Bintari — 2024-12-16

The natural course of the disease in patients with urinary tract infections (UTIs) is often unpredictable, so a proper diagnosis is needed through accurate laboratory tests. Urine specimen management is important for laboratory staff to pay attention to because delays in examination risk increasing false positive or negative results. The purpose of this study was to determine whether there was an effect of delaying the examination of urine specimens stored in a cool box on the viability of bacteria from urine culture examination results. The type of research was experimental, conducted at the Bacteriology Laboratory of STIKES Wira Medika Bali in June-August 2024. The experimental group consisted of delays in urine specimen examination for 0 hours, 2 hours, 4 hours and 6 hours. The calculation of bacterial viability was carried out by examining urine cultures with a calibrated loop technique. The research data were analyzed using the Kruskal Wallis bivariate test. The results showed that there was no significant difference in the viability of bacteria from urine culture at delays of 0 hours, 2 hours, 4 hours and 6 hours (p> 0.05) stored in a cool box. Urine specimens for calculating bacterial viability were still stable in cool box storage up to a delay time of 6 hours. It is suggested that further research is needed to determine whether there is a difference in bacterial viability in specimens stored in a cool box with a delay time of > 6 hours.

UJI ANTIMIKROBA EKSTRAK DAUN CIPLUKAN (Physalis angulata L.) TERHADAP PERTUMBUHAN BAKTERI Streptococcus pyogenes

Anas Fadli Wijaya — 2024-12-16

Infectious diseases caused by microbes in Indonesia are mostly due to bacterial infections. One of the bacteria responsible is Streptococcus pyogenes, which causes Acute Respiratory Infections. Treatment of infections caused by S. pyogenes mostly involves the use of antibiotics. In some cases, antibiotics often lead to resistance, necessitating alternative solutions, such as antimicrobial compounds derived from natural sources like flavonoids, one of which can be found in the extract of ground cherry leaf (Physalis angulata L.). The aim of this study is to investigate the antibacterial effects of a 70% ethanol extract of ground cherry leaves on the growth of S. pyogenes. This research is an experimental laboratory study using a post-test control group design only. The determination of antimicrobial activity was carried out using the well diffusion method. The ground cherry leaf extract, diluted with 10% DMSO, was pipetted into the wells. The positive control used chloramphenicol, while the negative control used 10% DMSO. Based on the observations, an inhibition zone was formed in the positive control with an average of 26.18 Â± 1.88 mm, treatment 1 with 100% ground cherry leaf extract 16.22 Â± 1.33 mm, treatment 2 with 50% ciplukan leaf extract 15.09 Â± 1.01 mm, and treatment 3 with 25% ground cherry leaf extract 12.85 Â± 0.87 mm.

ANALISIS KADAR ASAM LAKTAT DENGAN INTENSITAS LOW BACK PAIN PADA PEMANEN SAWIT PT X KABUPATEN KAMPAR

yuharika pratiwi — 2024-12-18

Background: Repetitive movements and poor body posture during work often cause low back pain (LBP), which radiates pain from the corner of the lowest ribs to the corner of the buttocks in the lumbar-sacral area. The prevalence of LBP ranges between 60 - 80% in the world. Risk factors for LBP in workers are excessive workload on the bones, which causes injury or trauma to soft tissue. Palm harvesters often experience muscle tension due to awkward postures for long periods, resulting in increased lactic acid levels, which are related to the intensity of LBP. An initial survey of PT Objective: To determine the analysis of the relationship between lactic acid levels and low back pain in palm oil harvesters at PT. X Kampar Regency Method: This research is an observational analytic, cross-sectional design. The sampling technique uses total sampling, with statistical tests using the Spearman test. Results: The results of the Spearman correlation test showed that p-value = 0.000; r 0.649 with a strong positive relationship strength. Conclusion: There is a relationship between lactic acid levels and LBP intensity in PT palm oil harvesters. X.