HASIL PEMERIKSAAN KOLESTEROL TOTAL PADA SERUM SEGERA DIPERIKSA DAN DITUNDA 7 HARI PADA SUHU 2 - 8ºC

RESULTS OF TOTAL CHOLESTEROL EXAMINATION IN THE IMMEDIATE SERUM CHECKED AND DELAYED 7 DAYS AT TEMPERATURE 2-8ºC

  • Karneli Karneli Poltekkes Kemenkes Palembang
  • Alfina Amelda
  • Asrori Asrori

Abstract

Background: Lipid profile examination that is often done in a laboratory is an examination of total cholesterol, which can be done by using a serum specimen. Although it is highly recommended to do a direct serum check, there are a number of reasons for delaying the examination, such as a broken device, or a power outage that makes the examination must be postponed. Serum that is not immediately checked can be stored. Storage of specimens is one of the pre-analytic stages, which has a significant risk of error in a laboratory examination. Objectives: This study aims to determine the comparison of the results of total cholesterol in serum which is immediately checked and postponed for 7 days at a temperature of 2-8ºC. Methods: This research was an experimental study with one group pre and posttest design. The population of this study were all Level 1 and 2 Students of Health Analyst Department of Health Ministry of Health, Palembang in 2020, totaling 150 people. The sample of this study was serum from 30 respondents taken by simple random sampling. Total cholesterol levels were checked using the Biosystem bts-350. Results: From the Shapiro Wilk data normality test obtained data with normal distribution. Data analysis continued with t dependent test, p = 0.403 was obtained, it means that p> α (0.05), which means there was no difference in the results of the examination of total cholesterol levels in serum that was immediately examined and stored for 7 days at a temperature of 2-8ºC. Conclusion: It can be concluded that there is no significant difference in the results of the examination of total cholesterol levels in serum that are immediately examined and stored for 7 days at a temperature of 2-8ºC.

References

Agustin, Y. (2018) ‘Perbandingan Hasil Pemeriksaan Glukosa Darah Sewaktu Pada Serum Yang Segera Diperiksa Dan Yang Ditunda Selama 2 Jam Menggunakan Tabung Vakum Gel Separator Tahun 2018’, Poltekkes Kemenkes Palembang.
Nugraha, G. (2017) Panduan Pemeriksaan Laboratorium Hematologi Dasar. Jakarta: TIM.

PERMENKES (2013) ‘PERMENKES RI NO. 43 TAHUN 2013 Tentang Cara Penyelenggaraan Laboratorium Klinik Yang Baik’, KEMENKES RI.

Plebani, M. (2006) Errors in Clinical Laboratories or Errors in Laboratory Medicine. Available at: https://www.ncbi.nlm.nih.gov/pubmed/16729864 (Accessed: 5 January 2020).

Purbayanti, D. (2015) ‘Pengaruh Waktu Pada Penyimpanan Serum Untuk Pemeriksaan Kolesterol Total’, Jurnal Surya Medika, 1(1).
Sadikin, M. (2002) Biokimia Enzim. Jakarta: Widya Medika.
SNI ISO 15189 (2012) Laboratorium medik – Persyaratan mutu dan kompetensi. Jakarta: Badan Standardisasi Nasional (BSN).
Published
2020-12-21
Section
Articles
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