IDENTIFICATION WITH Ascaris lumbricoides EGG USING TRICHROME STAIN
Abstract
The trichrome staining technique on stool specimens is a modification of the Gomori staining technique and has since been used as a diagnostic tool for the identification of intestinal parasites. The purpose of this examination was to determine the morphology of Ascaris lumbricoides using trichrome staining. The faecal examination method used is the direct preparation method by dropping the stool specimen on a glass slide which is then stained with trichrome or 2% Eosin and then covered with a cover slip, after which it is examined under a microscope. The results of trichrome staining on the color and morphology of worm eggs were better than using 2% Eosin. In trichrome staining made of 4 preparations, fertilized eggs were found with an oval shape, measuring 45 - 75 microns long and 35 - 50 microns wide, with a bright green background, giving the eggs a brownish yellow color with a dark green coating, so that the parts of the egg are clearly visible, while in Eosin 2% it gives a brownish red color to the egg and has a contrasting red background so that the parts of the egg are rather difficult to distinguish.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Please find the rights and licenses in Jurnal Ilmu Kesehatan Abdurrab. By submitting the article/manuscript of the article, the author(s) accept this policy.
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
2. Author’s Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Jurnal Ilmu Kesehatan Abdurrab spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, Jurnal Ilmu Kesehatan Abdurrab permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and Jurnal Ilmu Kesehatan Abdurrab on distributing works in the journal.
4. Rights of Authors
Authors retain all their rights to the published works, such as (but not limited to) the following rights;
- Copyright and other proprietary rights relating to the article, such as patent rights,
- The right to use the substance of the article in own future works, including lectures and books,
- The right to reproduce the article for own purposes,
- The right to self-archive the article,
- The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal (Jurnal Ilmu Kesehatan Abdurrab).
5. Co-Authorship
If the article was jointly prepared by other authors, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. Jurnal Ilmu Kesehatan Abdurrab will not be held liable for anything that may arise due to the author(s) internal dispute. Jurnal Ilmu Kesehatan Abdurrab will only communicate with the corresponding author.
6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by Jurnal Ilmu Kesehatan Abdurrab.
7. Miscellaneous
Jurnal Ilmu Kesehatan Abdurrab will publish the article (or have it published) in the journal if the article’s editorial process is successfully completed. Jurnal Ilmu Kesehatan Abdurrab's editors may modify the article to a style of punctuation, spelling, capitalization, referencing and usage that deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers as mentioned in point 3.
PDF
Abstract views: 136
downloads: 109
