IDENTIFIKASI TELUR Ascaris lumbricoides DENGAN MENGGUNAKAN PEWARNAAN TRICHOROME
Kata Kunci:
Morfologi, Telur, Ascaris lumbricoides, TrichromeAbstrak
Teknik pewarnaan trichrome pada specimen feses merupakan modifikasi dari Teknik pewarnaan Gomori dan sejak saat itu digunakan sebagai alat diagnostik untuk identifikasi parasit usus. Tujuan dari pemeriksaan ini adalah untuk mengetahui morfologi telur Ascaris lumbricoides dengan menggunakan pewarnaan trichrome. Metode pemeriksaan feses yang digunakan adalah metode sediaan langsung dengan cara spesimen feses diteteskan pada kaca preparat yang kemudian diberi pewarnaan trichrome atau Eosin 2% lalu di tutup dengan menggunakan kaca penutup, setelah itu diperiksa di bawah mikroskop. Hasil pewarnaan dari trichrome terhadap warna dan morfologi telur cacing lebih baik dibandingkan dengan menggunakan Eosin 2%. Pada pewarnaan trichrome yang dibuat sebanyak 4 preparat ditemukan telur yang sudah dibuahi dengan bentuk oval, berukuran panjang 45 - 75 mikron dan lebar 35 – 50 mikron, dengan latar belakang yang berwarna hijau terang, memberikan warna kuning kecoklatan pada telur dengan lapisan berwarna hijau tua, sehingga bagian-bagian pada telur terlihat jelas, sedangkan pada Eosin 2% memberikan warna merah kecoklatan pada telur dan memiliki latar belakang merah yang kontras sehingga bagian-bagian telur agak sulit di bedakan.
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