Uji Efektivitas Mukolitik Ramuan Buah Adas (Foeniculum vulgare Mill.) Secara In Vitro pada Putih Telur Bebek

Mucolytic Effectiveness Test of Fennel Fruit Concoction (Foeniculum vulgare Mill.) in Vitro on Duck Egg White

Abstract

Fennel (Foeniculum vulgare) empirically has been widely used by the community as a cough medicine with added honey. This study aims to determine the mucolytic activity of fennel herb in vitro on duck egg white with a concentration of 10% and 20%. A Fennel fruit concoction is obtained by brewing it with hot water and adding a teaspoon of honey. Concoctions of 10% and 20% fennel fruit were added with tween 80 and a mixture of duck egg white and phosphate buffer solution pH 7. Ambroxol HCl 3% was used as a positive control while mucus without boiling fennel was used as a negative control. The sample was tested using an Ostwald viscometer and its density was measured with a pycnometer. The data obtained is then processed to obtain the viscosity value. The results were analyzed by a one-way ANOVA statistical test and continued with the LSD test. The result showed that there was a significant difference between the negative control and the positive control, with concentrations of 10% and 20% (p < 0.05) and there was no significant difference between the positive controls with concentrations of 10% and 20% (p > 0.05). From this study, it can be concluded that the decoction of fennel fruit with added honey is proven to have mucolytic activity at concentrations of 10% and 20%.