Toxicity Test of 96% Ethanol Extract of Mahkota Dewa Leaves (Phaleria Macrocarpa) using the Brine Shrimp Lethality Test Method

  • Larysa Fernenda Universitas Abdurrab
  • Kony Putriani Universitas Abdurrab
  • Oksi Apriliani Universitas Abdurrab

Abstract

Mahkota dewa (Phaleria macrocarpa) is a plant from the Thymelaeceae family. This plant contains several active compounds such as alkaloids, flavonoids, steroids, saponins, and tannins, so it has potential as a herbal medicine in Indonesia. This study aims to determine the toxicity of the leaves of the god crown using the Brine Shrimp Lethality Test (BSLT) method. Extraction of the god crown leaves using 96% ethanol solvent with the maceration method was then carried out a phytochemical screening which showed that the sample of the crown god leaf contained alkaloids, flavonoids, steroids, saponins and tannins. Toxicity testing of the ethanol extract of Mahkota Dewa leaves on Artemia salina Leach larvae was divided into 5 test groups, namely 4 treatment groups (concentrations of 250 ppm, 500 ppm, 750 ppm, and 1000 ppm) and 1 control or comparison group (seawater). Each concentration was made in 3 vials including 10 Artemia salina Leach larvae. Observations were made by looking at the death of Artemia salina Leach larvae after 24 hours of treatment. The research results can be seen through probit analysis by calculating the LC50 value. The LC50 value of the ethanol extract of Mahkota Dewa leaves was 7,7090 /mL. This shows that the ethanol extract of Mahkota Dewa leaves has a toxic effect on Artemia salina Leach larvae because the LC50 value is <1000 ppm.

 

Keywords: Phaleria macrocarpa, Acute Toxicity Test, Artemia salina Leach, LC50.
Published
2024-08-13
How to Cite
Fernenda, L., Putriani, K., & Apriliani, O. (2024). Toxicity Test of 96% Ethanol Extract of Mahkota Dewa Leaves (Phaleria Macrocarpa) using the Brine Shrimp Lethality Test Method. Jurnal Ilmu Kesehatan Abdurrab, 2(1), 10-17. Retrieved from https://jurnal.univrab.ac.id/index.php/jika/article/view/4941
Issue
Vol 2 No 1 (2024): Volume 2 Nomor 1 Maret 2024
Section
Articles
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