Uji Toksisitas Ekstrak Etanol 96% Daun Mahkota Dewa (Phaleria Macrocarpa) dengan Metode Brine Shrimpe Lethality Test (BSLT)

Penulis

  • Larysa Fernenda Universitas Abdurrab
  • Kony Putriani Universitas Abdurrab
  • Oksi Apriliani Universitas Abdurrab

Kata Kunci:

Phaleria macrocarpa,Uji Toksisitas Akut, Artemia salina Leach, LC50

Abstrak

Mahkota dewa (Phaleria macrocarpa) merupakan tumbuhan dari famili Thymelaeceae. Tanaman ini mengandung beberapa senyawa aktif seperti alkaloid, flavonoid, steroid, saponin, tanin sehingga memiliki potensi sebagai obat herbal di indonesia. Penelitian ini bertujuan untuk mengetahui toksisitas dari daun mahkota dewa dengan menggunakan metode Brine Shrimp Lethality Test (BSLT). Estraksi daun mahkota dewa menggunakan pelarut etanol 96% dengan metode maserasi kemudian dilakukan skrining fitokimia yang menunjukkan sampel daun mahkota dewa mengandung senyawa alkaloid, flavonoid, steroid, saponin dan tanin. Pengujian toksisitas ekstrak etanol daun mahkota dewa terhadap larva Artemia salina Leach dibagi menjadi 5 kelompok uji yaitu 4 kelompok perlakuan (konsentrasi 250 ppm, 500 ppm, 750 ppm, dan 1000 ppm) dan 1 kelompok kontrol atau pembanding (air laut). Masing-masing konsentrasi dibuat dalam 3 vial yang dimasukkan 10 ekor larva Artemia salina Leach. Pengamatan dilakukan dengan melihat kematian larva Artemia salina Leach setelah perlakuan selama 24 jam. Hasil penelitian dapat dilihat melalui analisa probit dengan menghitung nilai LC50. Nilai LC50 dari ekstrak etanol daun mahkota dewa adalah 7,7090 /mL. Hal tersebut menunjukkan bahwa ekstrak etanol daun mahkota dewa memiliki efek toksik terhadap larva Artemia salina Leach karena nilai LC50 <1000 ppm.

Unduhan

Data unduhan belum tersedia.

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Diterbitkan

2024-08-13

Cara Mengutip

Fernenda, L., Putriani, K., & Apriliani, O. (2024). Uji Toksisitas Ekstrak Etanol 96% Daun Mahkota Dewa (Phaleria Macrocarpa) dengan Metode Brine Shrimpe Lethality Test (BSLT). Jurnal Ilmu Kesehatan Abdurrab, 2(1), 10–17. Diambil dari https://jurnal.univrab.ac.id/index.php/jika/article/view/4941

Terbitan

Vol 2 No 1 (2024): Volume 2 Nomor 1 Maret 2024

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