Determination of Total Flavonoid Content of Limpasu Pericarp Extract (Baccaurea lanceolata) by Spectrophotometric Uv-Vis

Risa Ahdyani

doi:10.36341/jops.v6i1.3014

Risa Ahdyani Universitas Muhamamdiyah Banjarmasin

: https://doi.org/10.36341/jops.v6i1.3014

Abstract

Limpasu (Baccaurea lanceolata) is a native plant of Borneo which is used empirically to protect the skin from sun exposure. Limpasu contains flavonoid compounds which generally act as antioxidants. This study aims to determine the total flavonoid content of limpasu pericarp extract by using UV-Vis spectrophotometry method. Limpasu pericarp was extracted by using the stratification maceration method. This extraction was carried out by immersing the simplicia into three types of solvents with different polarities, respectively, namely n-hexane (nonpolar), ethyl acetate (semi-polar), and methanol (polar). The concentration of n-hexane, ethyl acetate, and methanol extracts was determined using a UV-Vis spectrophotometer at a maximum wavelength of 510 nm with quercetin as standard. Based on the test, it was found that the total flavonoid content of pericarp extracts, hexane, ethyl acetate, and methanol, respectively, was 44.1552 g/ml; 155.8506 g/ml; and 25.8506 g/ml. The highest total flavonoid content was found in the ethyl acetate extract.

Keywords: Limpasu, Maceration, Extract, Flavonoid

References

Abu Bakar, M. F., Ahmad, N. E., Karim, F. A., & Saib, S. (2014). Phytochemicals and antioxidative properties of borneo indigenous liposu (Baccaurea lanceolata) and tampoi (Baccaurea macrocarpa) fruits. Antioxidants, 3(3), 516–525. https://doi.org/10.3390/antiox3030516
Alifni, *, Bakti, A., Triyasmono, L., & Rizki, M. I. (2017). Penentuan Kadar Flavonoid Total dan Uji Antioksidan Ekstrak Etanol Daun Kasturi (Mangifera casturi Kosterm.) dengan Metode DPPH. Jurnal Pharmascience, 04(01), 102–108. http://jps.unlam.ac.id/
Asri Widyasanti, D. N. M. D. R. (2019). KARAKTERISTIK MUTU EKSTRAK TEH PUTIH(Camellia sinensis)YANG DIHASILKAN DARI METODE MASERASI BERTINGKAT DENGANPELARUT n-HEKSANA, ASETON 70%, DAN ETANOL 96%. Jurnal Teknik Pertanian Lampung, 8(4), 293–299.
Fauzana, A., & Wardaniati, I. (2022). Determination of Vitamin C Content in Fresh Ananas comocus L. Fruit Cultivated in Teluk Meranti District, Pelalawan using Spectrophotometry UV-Vis Method Penetapan Kadar Vitamin C Buah Nanas Segar (Ananas comocus L.) Hasil Budidaya di Kecamatan Teluk Meranti, Kabupaten Pelalawan dengan Metode Spektrofotometri UV-Vis. Journal Of Pharmacy and Science), 5(2), 54–61.
Fitriansyah, S., Putri, Y. D., Aulifa, D. L., Haris, M., Agustina, Y., & Firman. (2018). AKTIVITAS ANTIOKSIDAN, TOTAL FENOLIK DAN TOTAL FLAVONOID EKSTRAK BUAH, DAUN DAN KULIT BATANG LIMPASU ( Baccaurea lanceolata ). Jurnal Farmasi Galenika, 5(3), 115–121.
Fitriansyah, S., Putri, Y. D., Haris, M., Ferdiansyah, R., Nurhayati, R., Sari, Y., Purnama, & Usni. (2018). AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH, DAUN, DAN KULIT BATANG LIMPASU (Baccaurea lanceolata (Miq.) Müll.Arg.) dari. Pharmaceutical Journal of Indonesia, 15(02), 1–21.
Hadi, S., Mangkurat, U. L., Wahyuono, S., & Mada. (2018). EARCH ACTIVE FRACTION OF Baccaurea lanceolate TAPIN DISTRICT, SOUTH KALIMANTAN AS ANTIOXIDANTS. PHARMACY, 12(2), 242–246.
Hadi, S., Wahyuono, S., Yuswanto, A., & Lukitaningsih, E. (2017). SPF Test from Baccaurea lanceolata Muell . Arg Fruit Isolates. Indonesian Journal of Cancer Chemoprevention, 8(1), 38–41. https://doi.org/10.14499/indonesianjcanchemoprev8iss1pp38-41
Pourmorad, F., Hosseinimehr, S. J., & Shahabimajd, N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology, 5(11), 1142–1145.
Rahman, A., & Taufiqurrahman, I. (2017). PERBEDAAN TOTAL FLAVONOID ANTARA METODE MASERASI DENGAN SOKLETASI PADA EKSTRAK DAUN RAMANIA (Bouea macrophylla Griff) (Studi pendahuluan terhadap proses pembuatan sediaan obat penyembuhan luka) (Issue 1).
Ratnapuri, P. H., Haitami, F., & Fitriana, M. (2019). Stabilitas Fisik Sediaan Emulgel Ekstrak Etanol Daging Buah Limpasu (Baccaurea lanceolata (Miq.) Müll. Arg.). Jurnal Pharmascience, 06(02), 8–18. https://ppjp.ulm.ac.id/journal/index.php/pharmascience
Samsul Hadi, S. W. Ag. Y. RR. E. L. (2015). PENELUSURAN FRAKSI AKTIF Baccaurea lanceolata DARI KABUPATEN TAPIN, KALIMANTAN SELATAN SEBAGAI ANTIOKSIDAN. Pharmacy, 12(2), 242–246.
Tenri Ugi Dg Pine Jurusan Farmasi Fakultas Kedokteran dan Ilmu Kesehatan UIN Alauddin Makassar, A. (2016). Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Bidara (Ziziphus spina-christi L.). In Journal of Pharmaceutical and Medicinal Sciences (Vol. 1, Issue 2).
Toar Waraney Senduk, L. A. D. Y. M. V. D. (2020). ENDEMEN EKSTRAK AIR REBUSAN DAUN TUA MANGROVE Sonneratia alba (The rendement of boiled water extract of mature leaves of mangrove Sonneratia alba). E-ISSN :2302-6081 p-ISSN 2302-609X Jurnal Perikanan Dan Kelautan Tropis, 11(1), 9–15.
Uluk, A., Sudana, M., & Wollenberg, E. (2001). Ketergantungan Masyarakat Dayak Terhadap Hutan. Center for International Forestry Research.
Zamzani, I., & Triadisti, N. (2021). Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method. Jurnal Profesi Medika : Jurnal Kedokteran Dan Kesehatan, 15(1), 60–68. https://doi.org/10.33533/jpm.v15i1.2820